TIMP1 ELISA 试剂盒
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- 抗原 See all TIMP1 ELISA试剂盒
- TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 30-18.000 pg/mL
- 最低检测浓度
- 30 pg/mL
- 应用范围
- ELISA
- 原理
- Human TIMP-1 ELISA Kit for cell and tissue lysate samples.
- 样品类型
- Tissue Lysate, Cell Lysate
- Analytical Method
- Quantitative
- 特异性
- The antibody pair provided in this kit recognizes human TIMP-1.
- 灵敏度
- 30 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Cell lysate buffer
- Featured
- Discover our best selling TIMP1 ELISA Kit
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- Discover our top product TIMP1 ELISA Kit
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TIMP Metallopeptidase Inhibitor 1 (TIMP1) ELISA Kit
TIMP1 适用: 人 Colorimetric Sandwich ELISA 0.39-25 ng/mL Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 1 (TIMP1) ELISA KitTIMP1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 1 (TIMP1) ELISA KitTIMP1 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 1 (TIMP1) ELISA KitTIMP1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 1 (TIMP1) ELISA KitTIMP1 适用: 犬 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 1 (TIMP1) ELISA KitTIMP1 适用: 人 AA 24-207 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Saliva, Serum
TIMP Metallopeptidase Inhibitor 1 (TIMP1) ELISA KitTIMP1 适用: 兔 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 1 (TIMP1) ELISA KitTIMP1 适用: 豚鼠 Colorimetric Sandwich ELISA 4.69 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 1 (TIMP1) ELISA KitTIMP1 适用: 绵羊 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
TIMP Metallopeptidase Inhibitor 1 (TIMP1) ELISA KitTIMP1 适用: 兔 Colorimetric Competition ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
- 抗原 See all TIMP1 ELISA试剂盒
- TIMP1 (TIMP Metallopeptidase Inhibitor 1 (TIMP1))
- 别名
- TIMP-1 (TIMP1 产品)
- 别名
- TIMP-1 ELISA Kit, TIMP1 ELISA Kit, DKFZp468A0912 ELISA Kit, CLGI ELISA Kit, EPA ELISA Kit, EPO ELISA Kit, HCI ELISA Kit, TIMP ELISA Kit, Timp ELISA Kit, Clgi ELISA Kit, TIMP metallopeptidase inhibitor 1 ELISA Kit, tissue inhibitor of metalloproteinase 1 ELISA Kit, TIMP1 ELISA Kit, Timp1 ELISA Kit
- 背景
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Gene Names: TIMP1 CLGI TIMP
Protein names: Metalloproteinase inhibitor 1 (Erythroid-potentiating activity) (EPA) (Fibroblast collagenase inhibitor) (Collagenase inhibitor) (Tissue inhibitor of metalloproteinases 1) (TIMP-1)
- 基因ID
- 7076
- UniProt
- P01033
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