MMP13 ELISA 试剂盒
(8 references)Quick Overview for MMP13 ELISA 试剂盒 (ABIN625055)
抗原
See all MMP13 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 6 pg/mL
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原理
- Human MMP-13 ELISA Kit for cell culture supernatants, heparin treated plasma, and serum samples. EDTA and Citrate are not recommended.
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Analytical Method
- Quantitative
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特异性
- This ELISA kit shows no cross-reactivity with any of the cytokines tested Human Angiopoietin-1, Angiostatin , B7-1, BMP-7, CD14, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4 , DR6, Endostatin, E-Selectin, Follistatin, HB-EGF, HVEM, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-18, IL-9 , IL-2 Ra, IL-2 Rb, IL-5 Ra, LAP, L-Selectin, M-CSF R, MMP-1, 2, 3, 7, 8, 9, 10 and 12, PDGF-AB, SDF-1b, Tie-1, Tie-2, TIMP-3.
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灵敏度
- 6 pg/mL
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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SensoLyte® 520 MMP-13 Assay Kit
FRET Fluorometric
SensoLyte® Plus 520 MMP-13 Assay Kit Fluorimetric and Enhanced SelectivityFRET 适用: 人 Fluorometric
SensoLyte® 490 MMP-13 Assay KitFRET Fluorometric
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 兔 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 人 AA 20-471 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metallopeptidase 13 (Collagenase 3) (MMP13) ELISA KitMMP13 适用: 小鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Recommended Dilution for serum and plasma samples2 fold
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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样品制备
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Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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: "Expression and pathological effects of periostin in human osteoarthritis cartilage." in: BMC musculoskeletal disorders, Vol. 16, pp. 215, (2015) (PubMed).
: "Maternal plasma prolidase, matrix metalloproteinases 1 and 13, and oxidative stress levels in pregnancies complicated by preterm premature rupture of the membranes and chorioamnionitis." in: Journal of the Turkish German Gynecological Association, Vol. 13, Issue 3, pp. 172-7, (2014) (PubMed).
: "Baicalein ameliorates inflammatory-related apoptotic and catabolic phenotypes in human chondrocytes." in: International immunopharmacology, Vol. 21, Issue 2, pp. 301-8, (2014) (PubMed).
: "Effects of eye rubbing on the levels of protease, protease activity and cytokines in tears: relevance in keratoconus." in: Clinical & experimental optometry : journal of the Australian Optometrical Association, Vol. 96, Issue 2, pp. 214-8, (2013) (PubMed).
: "Association of endothelin-1 expression and cartilaginous endplate degeneration in humans." in: PLoS ONE, Vol. 8, Issue 4, pp. e60062, (2013) (PubMed).
: "Stimulation of matrix metalloproteinases by tumor necrosis factor-? in human pulp cell cultures." in: Journal of endodontics, Vol. 39, Issue 6, pp. 795-800, (2013) (PubMed).
: "Caesalpinia sappan extract inhibits IL1?-mediated overexpression of matrix metalloproteinases in human chondrocytes." in: Genes & nutrition, Vol. 7, Issue 2, pp. 307-18, (2012) (PubMed).
: "Matrix metalloproteinase levels as a marker for rotator cuff tears." in: Orthopedics, Vol. 35, Issue 4, pp. e474-8, (2012) (PubMed).
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: "Expression and pathological effects of periostin in human osteoarthritis cartilage." in: BMC musculoskeletal disorders, Vol. 16, pp. 215, (2015) (PubMed).
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- MMP13 (Matrix Metallopeptidase 13 (Collagenase 3) (MMP13))
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别名
- MMP-13
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背景
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Gene Names: MMP13
Protein names: Collagenase 3 (EC 3.4.24.-) (Matrix metalloproteinase-13) (MMP-13)
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基因ID
- 4322
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UniProt
- P45452
抗原 See all MMP13 ELISA试剂盒
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