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MMP1 ELISA 试剂盒
MMP1
适用: 人
Colorimetric
Sandwich ELISA
8-18000 pg/mL
Cell Lysate, Tissue Lysate
MMP1ELISA Kit详情
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MMP1
(Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))
适用
All reactivities for MMP1 ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
8-18000 pg/mL
最低检测浓度
8 pg/mL
应用范围
ELISA
原理
Human MMP-1 ELISA Kit for cell and tissue lysate samples.
样品类型
Tissue Lysate, Cell Lysate
Analytical Method
Quantitative
特异性
The antibody pair provided in this kit recognizes human MMP-1.
灵敏度
8 pg/mL
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis Cell lysate buffer
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使用细节
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样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
MMP1目标详情
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MMP1
(Matrix Metallopeptidase 1 (Interstitial Collagenase) (MMP1))
别名
MMP-1 (MMP1 产品 )
别名
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背景
Gene Names: MMP1 CLG Protein names: Interstitial collagenase (EC 3.4.24.7) (Fibroblast collagenase) (Matrix metalloproteinase-1) (MMP-1) [Cleaved into: 22 kDa interstitial collagenase, 27 kDa interstitial collagenase]
基因ID
4312
UniProt
P03956
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