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CCL19 ELISA 试剂盒
CCL19
适用: 人
Colorimetric
Sandwich ELISA
2-500 pg/mL
Cell Culture Supernatant, Plasma, Serum
CCL19ELISA Kit详情
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CCL19
(Chemokine (C-C Motif) Ligand 19 (CCL19))
适用
All reactivities for CCL19 ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
2-500 pg/mL
最低检测浓度
2 pg/mL
应用范围
ELISA
原理
Human MIP-3 beta (CCL19) ELISA Kit for cell culture supernatants, plasma, and serum samples.
样品类型
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
特异性
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
灵敏度
2 pg/mL
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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使用细节
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应用备注
Recommended Dilution for serum and plasma samples2 - 10 fold
样本量
100 μL
板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
-20 °C
储存方法
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
有效期
6 months
CCL19目标详情
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抗原
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CCL19
(Chemokine (C-C Motif) Ligand 19 (CCL19))
别名
MIP-3 beta (CCL19 产品 )
别名
CKb11 ELISA Kit, ELC ELISA Kit, MIP-3b ELISA Kit, MIP3B ELISA Kit, SCYA19 ELISA Kit, CCL19 ELISA Kit, Scya19 ELISA Kit, exodus-3 ELISA Kit, C-C motif chemokine ligand 19 ELISA Kit, chemokine (C-C motif) ligand 19 ELISA Kit, CCL19 ELISA Kit, ccl19 ELISA Kit, Ccl19 ELISA Kit
背景
Gene Names: CCL19 ELC MIP3B SCYA19 Protein names: C-C motif chemokine 19 (Beta-chemokine exodus-3) (CK beta-11) (Epstein-Barr virus-induced molecule 1 ligand chemokine) (EBI1 ligand chemokine) (ELC) (Macrophage inflammatory protein 3 beta) (MIP-3-beta) (Small-inducible cytokine A19)
基因ID
6363
UniProt
Q99731
途径
Positive Regulation of Immune Effector Process
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