CCL1 ELISA 试剂盒
(3 references)-
- 抗原 See all CCL1 ELISA试剂盒
- CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))
-
适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 4-3000 pg/mL
- 最低检测浓度
- 4 pg/mL
- 应用范围
- ELISA
- 原理
- Human I-309 (TCA-3/CCL1) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
- 灵敏度
- 4 pg/mL
- 产品特性
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Featured
- Discover our best selling CCL1 ELISA Kit
- Top Product
- Discover our top product CCL1 ELISA Kit
-
Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA Kit
CCL1 适用: 人 Colorimetric Sandwich ELISA 312 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA KitCCL1 适用: 大鼠 Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA KitCCL1 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA KitCCL1 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum
Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA KitCCL1 适用: 小鼠 AA 24-92 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA KitCCL1 适用: 人 AA 24-96 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA KitCCL1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA KitCCL1 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
-
- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
-
- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
-
-
: "Tumor cell entry into the lymph node is controlled by CCL1 chemokine expressed by lymph node lymphatic sinuses." in: The Journal of experimental medicine, Vol. 210, Issue 8, pp. 1509-28, (2013) (PubMed).
: "Effect of serum amyloid A1 treatment on global gene expression in THP-1-derived macrophages." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 61, Issue 4, pp. 391-8, (2012) (PubMed).
: "Irradiated Mesenchymal Stem Cells improve the ex vivo expansion of Hematopoietic Progenitors by partly mimicking the bone marrow endosteal environment." in: Journal of immunological methods, Vol. 370, Issue 1-2, pp. 93-103, (2011) (PubMed).
-
: "Tumor cell entry into the lymph node is controlled by CCL1 chemokine expressed by lymph node lymphatic sinuses." in: The Journal of experimental medicine, Vol. 210, Issue 8, pp. 1509-28, (2013) (PubMed).
-
- 抗原 See all CCL1 ELISA试剂盒
- CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))
- 别名
- I-309 (CCL1 产品)
- 别名
- I-309 ELISA Kit, P500 ELISA Kit, SCYA1 ELISA Kit, SISe ELISA Kit, TCA3 ELISA Kit, BF534335 ELISA Kit, Scya1 ELISA Kit, Tca-3 ELISA Kit, CCLi6 ELISA Kit, chemokine ELISA Kit, C-C motif chemokine ligand 1 ELISA Kit, chemokine (C-C motif) ligand 1 ELISA Kit, CCL1 ELISA Kit, Ccl1 ELISA Kit
- 背景
-
Gene Names: CCL1 SCYA1
Protein names: C-C motif chemokine 1 (Small-inducible cytokine A1) (T lymphocyte-secreted protein I-309)
- 基因ID
- 6346
- UniProt
- P22362
-
