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CCL1 ELISA 试剂盒

Name: CCL1 ELISA 试剂盒
SKU: ABIN624994
Availability: OutOfStock

This 人 CCL1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 CCL1. This ELISA Kit has been cited in 3+ publications.

ELISA

1 image

(3 references)

产品编号 ABIN624994

发货至: 中国

Datasheet as PDF

Quick Overview for CCL1 ELISA 试剂盒 (ABIN624994)

抗原

See all CCL1 ELISA试剂盒

CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

4-3000 pg/mL

应用范围

ELISA

样品类型

Plasma, Cell Culture Supernatant, Serum

Discover our top product CCL1 ELISA Kit

最低检测浓度

4 pg/mL

原理

Human I-309 (TCA-3/CCL1) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytical Method

Quantitative

特异性

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, - 2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

灵敏度

4 pg/mL

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
试剂未包括
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA Kit
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Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA Kit
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CCL1 适用: 小鼠 AA 24-92 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA Kit
CCL1 适用: 人 AA 24-96 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum

Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA Kit
CCL1 适用: 小鼠 Colorimetric Sandwich ELISA 30-8000 pg/mL Cell Culture Supernatant, Plasma, Serum

Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA Kit
CCL1 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA Kit
CCL1 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Chemokine (C-C Motif) Ligand 1 (CCL1) ELISA Kit
CCL1 适用: 绵羊 Colorimetric Competition ELISA Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate

应用备注

Recommended Dilution for serum and plasma samples2 fold

样本量

100 μL

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
储存条件

-20 °C

储存方法

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

有效期

6 months
Das, Sarrou, Podgrabinska, Cassella, Mungamuri, Feirt, Gordon, Nagi, Wang, Entenberg, Condeelis, Skobe: "Tumor cell entry into the lymph node is controlled by CCL1 chemokine expressed by lymph node lymphatic sinuses." in: The Journal of experimental medicine, Vol. 210, Issue 8, pp. 1509-28, (2013) (PubMed).

Leow, Goh, Heng: "Effect of serum amyloid A1 treatment on global gene expression in THP-1-derived macrophages." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 61, Issue 4, pp. 391-8, (2012) (PubMed).

Celebi, Mantovani, Pineault: "Irradiated Mesenchymal Stem Cells improve the ex vivo expansion of Hematopoietic Progenitors by partly mimicking the bone marrow endosteal environment." in: Journal of immunological methods, Vol. 370, Issue 1-2, pp. 93-103, (2011) (PubMed).
抗原 See all CCL1 ELISA试剂盒

CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))

别名

I-309

背景

Gene Names: CCL1 SCYA1
Protein names: C-C motif chemokine 1 (Small-inducible cytokine A1) (T lymphocyte-secreted protein I-309)

基因ID

6346

UniProt

P22362