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Cyclin B1 ELISA 试剂盒

This 人 Cyclin B1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 Cyclin B1.

HeLa cells were untreated or treated with Nocodazole for 20 hours. (Cyclin B1 ELISA 试剂盒)

HeLa cells were untreated or treated with Nocodazole for 20 hours. (Cyclin B1 ELISA 试剂盒)

HeLa cells were treated with Nocodazole at 37°C for 20 hours. (Cyclin B1 ELISA 试剂盒)

3 images

产品编号 ABIN6244358

发货至: 中国

Quick Overview for Cyclin B1 ELISA 试剂盒 (ABIN6244358)

抗原

See all Cyclin B1 (CCNB1) ELISA试剂盒

Cyclin B1 (CCNB1)

抗原表位

pSer126, total

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

应用范围

ELISA

样品类型

Cell Samples, Tissue Lysate

Discover our top product CCNB1 ELISA Kit

原理

Human Phospho-Cyclin B1 (Ser126) and Total Cyclin B1 ELISA Kit. This assay semi-quantitatively measures Cyclin B1 phosphorylated at Serine-126 as well as total Cyclin B1 in cell lysate samples.

Analytical Method

Semi-Quantitative

特异性

This ELISA kit recognizes Human Cyclin B1 phosphorylated at site Serine-126 as well as total Cyclin B1.

产品特性
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Anti-Phospho Antibody
- Anti-Pan Antibody
- HRP-Conjugated Secondary Antibody
- Streptavidin-Conjugated HRP
- Assay Diluent
- TMB One-Step Substrate
- Stop Solution
- Lysis Buffer
- Positive Control Sample
试剂未包括
- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Protease and Phosphatase inhibitors
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
Cyclin B1 (CCNB1) ELISA Kit
CCNB1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum

Cyclin B1 (CCNB1) ELISA Kit
CCNB1 适用: 人 pSer126 Colorimetric Sandwich ELISA Cell Samples, Tissue Lysate

Cyclin B1 (CCNB1) ELISA Kit
CCNB1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate

Cyclin B1 (CCNB1) ELISA Kit
CCNB1 适用: 人, 小鼠, 大鼠 pSer126 Colorimetric Cell ELISA Cell Culture Cells

Cyclin B1 (CCNB1) ELISA Kit
CCNB1 适用: 人, 小鼠, 大鼠 pSer147 Colorimetric Cell ELISA Cell Culture Cells

应用备注

Optimal working dilution should be determined by the investigator.

板类型

Pre-coated

实验流程
1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
储存条件

-20 °C

储存方法

Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

有效期

6 months
抗原 See all Cyclin B1 (CCNB1) ELISA试剂盒

Cyclin B1 (CCNB1)

别名

Cyclin B1

基因ID

891

UniProt

P14635

途径

Cell Division Cycle, AMPK Signaling, Mitotic G1-G1/S Phases, M Phase

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