Adrenomedullin ELISA 试剂盒
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Quick Overview for Adrenomedullin ELISA 试剂盒 (ABIN6244353)
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See all Adrenomedullin (ADM) ELISA试剂盒适用
检测方法
实验类型
应用范围
样品类型
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原理
- Human/Mouse/Rat Adrenomedullin EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA
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Analytical Method
- Quantitative
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特异性
- This EIA kit is designed to detect human, mouse, and rat Adrenomedullin
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灵敏度
- 0.9 ng/mL
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
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Adrenomedullin (ADM) ELISA Kit
ADM 适用: 人 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adrenomedullin (ADM) ELISA KitADM 适用: 大鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adrenomedullin (ADM) ELISA KitADM 适用: 犬 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Adrenomedullin (ADM) ELISA KitADM 适用: 人 Colorimetric Sandwich ELISA 0.312 pg/mL - 20 pg/mL Plasma, Serum, Tissue Homogenate
Adrenomedullin (ADM) ELISA KitADM 适用: 人 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
Adrenomedullin (ADM) ELISA KitADM 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Adrenomedullin (ADM) ELISA KitADM 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Adrenomedullin (ADM) ELISA KitADM 适用: Cow Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Adrenomedullin (ADM) ELISA KitADM 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adrenomedullin (ADM) ELISA KitADM 适用: 犬 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
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应用备注
- Optimal working dilution should be determined by the investigator.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed.
- Add 100 μL detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4 °C.
- Add 100 μL standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 μL prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 μL TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL Stop Solution to each well.
- Read plate at 450 nm immediately.
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试剂准备
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Recommended Dilution for serum and plasma samplesHuman: 2x / Mouse: 2x / Rat: 2x
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
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有效期
- 6 months
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- Adrenomedullin (ADM)
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别名
- Adrenomedullin
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物质类
- Hormone
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背景
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Gene Symbols: ADM, AM
Background: Adrenomedullin (ADM or AM) consists of 52 amino acids, and is a potent hypotensive and vasodilatator agent. It was initially isolated in 1993 from a pheochromocytoma, a tumor of the adrenal medulla. ADM is found in blood in a considerable concentration and may function as a hormone related to the physiologic control of fluid and electrolyte homeostasis. It has also been reported that ADM stimulates the growth of new blood vessels (angiogenesis) and increasing the tolerance of cells to oxidative stress and hypoxic injury.
Protein Name (Synonyms): ADM [Cleaved into: Adrenomedullin (A
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基因ID
- 133
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途径
- Hormone Transport, Hormone Activity, C21-Steroid Hormone Metabolic Process, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Tube Formation
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