SFTPB ELISA 试剂盒
Quick Overview for SFTPB ELISA 试剂盒 (ABIN6232158)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 3.125 ng/mL
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原理
- Pig Pulmonary Surfactant-Associated Protein B (SFTPB) ELISA Kit
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Analytical Method
- Quantitative
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灵敏度
- 1.88 ng/mL
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组件
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The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- Pre-coated 96-Well Microplate
- Standard
- Standard Diluent Buffer
- Wash Buffer
- Detection Reagent A
- Detection Reagent B
- Diluent A
- Diluent B
- TMB Substrate
- Stop Solution
- Plate Sealer
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试剂未包括
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- 37 °C incubator
- Multi and single channel pipettes and sterile pipette tips
- Squirt bottle or automated microplate washer
- 1.5 mL tubes
- Distilled water
- Absorbent filter papers
- 100 mL and 1 liter graduated cylinders
- Microplate reader (wavelength: 450 nm)
- ELISA Shaker
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Surfactant Protein B (SFTPB) ELISA Kit
SFTPB 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Lung Lavage Fluid, Plasma, Serum, Tissue Homogenate
Surfactant Protein B (SFTPB) ELISA KitSFTPB 适用: 小鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Surfactant Protein B (SFTPB) ELISA KitSFTPB 适用: 大鼠 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Surfactant Protein B (SFTPB) ELISA KitSFTPB 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum, Urine
Surfactant Protein B (SFTPB) ELISA KitSFTPB 适用: 小鼠 Colorimetric Sandwich ELISA 0.45 ng/mL - 30 ng/mL Plasma, Serum, Tissue Homogenate
Surfactant Protein B (SFTPB) ELISA KitSFTPB 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Lung Lavage Fluid, Tissue Homogenate
Surfactant Protein B (SFTPB) ELISA KitSFTPB 适用: 兔 Colorimetric Sandwich ELISA 1.87 ng/mL - 120 ng/mL Plasma, Serum, Tissue Homogenate
Surfactant Protein B (SFTPB) ELISA KitSFTPB 适用: Cow Colorimetric Sandwich ELISA 9.37 ng/mL - 600 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal dilutions/concentrations should be determined by the end user.
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说明
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The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
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板类型
- Pre-coated
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Pulmonary Surfactant Associated Protein B / SP-B (SFTPB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Pulmonary Surfactant Associated Protein B / SP-B (SFTPB) were tested on 3 different plates, 8 replicates in each plate.
CV (%) = (Standard Deviation / mean) x 100
Intra-Assay: CV<10%
Inter-Assay: CV<10%
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.
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有效期
- 6 months
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- SFTPB (Surfactant Protein B (SFTPB))
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别名
- Pulmonary Surfactant-Associated Protein B
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背景
- A7MAK5_PIG
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基因ID
- 397296
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UniProt
- A7MAK5
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