SIRT5 ELISA 试剂盒
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Quick Overview for SIRT5 ELISA 试剂盒 (ABIN6210198)
抗原
See all SIRT5 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.156 ng/mL
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原理
- This kit is based on sandwich enzyme-linked immuno-sorbent assay technology.
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Analytical Method
- Quantitative
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组件
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The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- Pre-coated 96-Well Microplate
- Standard
- Standard Diluent Buffer
- Wash Buffer
- Detection Reagent A
- Detection Reagent B
- Diluent A
- Diluent B
- TMB Substrate
- Stop Solution
- Plate Sealer
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试剂未包括
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- 37 °C incubator
- Multi and single channel pipettes and sterile pipette tips
- Squirt bottle or automated microplate washer
- 1.5 mL tubes
- Distilled water
- Absorbent filter papers
- 100 mL and 1 liter graduated cylinders
- Microplate reader (wavelength: 450 nm)
- ELISA Shaker
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Sirtuin 5 (SIRT5) ELISA Kit
SIRT5 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Sirtuin 5 (SIRT5) ELISA KitSIRT5 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Sirtuin 5 (SIRT5) ELISA KitSIRT5 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Sirtuin 5 (SIRT5) ELISA KitSIRT5 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Tissue Homogenate
Sirtuin 5 (SIRT5) ELISA KitSIRT5 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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样本量
- 100 µL
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板类型
- Pre-coated
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实验流程
- This kit is based on sandwich enzyme-linked immuno-sorbent assay technology. An antibody is pre-coated onto a 96-well plate. Standards, test samples, and biotin-conjugated reagent are added to the wells and incubated. The HRP-conjugated reagent is then added, and the whole plate is incubated. Unbound conjugates are removed using wash buffer at each stage. TMB substrate is used to quantify the HRP enzymatic reaction. After TMB substrate is added, only wells that contain sufficient SIRT5 will produce a blue coloured product, which then changes to yellow after adding the acidic stop solution. The intensity of the yellow colour is proportional to the SIRT5 amount bound on the plate. The Optical Density (OD) is measured spectrophotometrically at 450 nm in a microplate reader, from which the concentration of SIRT5 can be calculated.
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试剂准备
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This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- 1) Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol.
- 2) Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol.
- 3) Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100.
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实验流程
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This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- 1) Set standard, test samples and control wells.
- 2) Aliquot 100 μL of diluted standard into the standard wells.
- 3) Aliquot 100 μL of Standard Diluent buffer into control (zero) well.
- 4) Aliquot 100 μL of diluted samples into the sample wells. Incubate for 1 hr at 37 °C.
- 5) Aliquot 100 μL of Detection Reagent A to each well. Incubate for 1 hr at 37 °C.
- 6) Wash 3 times.
- 7) Aliquot 100 μL of Detection Reagent B to each well. Incubate for 30 mins at 37 °C.
- 8) Wash 5 times.
- 9) Aliquot 90 μL of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C.
- 10) Aliquot 50 μL of Stop Solution.
- 11) Measure the OD at 450 nm.
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结果分析
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For calculation, average the O.D.450 duplicate readings for each reference standard and each sample and substract the average control (zero) O.D.450 reading. The standard curve can be plotted as the relative O.D.450 of each reference standard solution (Y) vs. the respective concentration of each standard solution (X). The SIRT5 concentration of the samples can be interpolated from the standard curve.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Shipped at 4°C. Upon receipt, store the kit according to the storage instruction in the kit's manual.The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
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有效期
- 6 months
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- SIRT5 (Sirtuin 5 (SIRT5))
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别名
- Sirtuin 5
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UniProt
- Q8K2C6
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途径
- SARS-CoV-2 Protein Interactome
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