Endothelin 1 ELISA 试剂盒
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Quick Overview for Endothelin 1 ELISA 试剂盒 (ABIN6205126)
抗原
See all Endothelin 1 (EDN1) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 1.25 pg/mL
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Analytical Method
- Quantitative
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灵敏度
- 0.75 pg/mL
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组件
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The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- Pre-coated 96-Well Microplate
- Standard
- Standard Diluent Buffer
- Wash Buffer
- Detection Reagent A
- Detection Reagent B
- Diluent A
- Diluent B
- TMB Substrate
- Stop Solution
- Plate Sealer
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试剂未包括
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- 37 °C incubator
- Multi and single channel pipettes and sterile pipette tips
- Squirt bottle or automated microplate washer
- 1.5 mL tubes
- Distilled water
- Absorbent filter papers
- 100 mL and 1 liter graduated cylinders
- Microplate reader (wavelength: 450 nm)
- ELISA Shaker
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Endothelin 1 (EDN1) ELISA Kit
EDN1 适用: 兔 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 大鼠 Colorimetric Sandwich ELISA 1.25 pg/mL - 80 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 人 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Saliva, Serum, Urine
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 豚鼠 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 小鼠 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 大鼠 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 人 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 适用: Pig Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 pg/mL - 50 pg/mL Cell Culture Supernatant, Plasma, Serum
Endothelin 1 (EDN1) ELISA KitEDN1 适用: 人 Colorimetric Sandwich ELISA 1.25 pg/mL - 80 pg/mL Cell Culture Supernatant, Plasma, Serum
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板类型
- Pre-coated
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实验流程
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This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- 1) Set standard, test samples and control wells.
- 2) Aliquot 100 μL of diluted standard into the standard wells.
- 3) Aliquot 100 μL of Standard Diluent buffer into control (zero) well.
- 4) Aliquot 100 μL of diluted samples into the sample wells. Incubate for 1 hr at 37 °C.
- 5) Aliquot 100 μL of Detection Reagent A to each well. Incubate for 1 hr at 37 °C.
- 6) Wash 3 times.
- 7) Aliquot 100 μL of Detection Reagent B to each well. Incubate for 30 mins at 37 °C.
- 8) Wash 5 times.
- 9) Aliquot 90 μL of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C.
- 10) Aliquot 50 μL of Stop Solution.
- 11) Measure the OD at 450 nm.
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Endothelin were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Endothelin were tested on 3 different plates, 8 replicates in each plate.
CV (%) = (Standard Deviation / mean) x 100
Intra-Assay: CV<10%
Inter-Assay: CV<10%
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
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有效期
- 6 months
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- Endothelin 1 (EDN1)
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别名
- Endothelin 1
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途径
- Hormone Transport, Negative Regulation of Hormone Secretion, Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of Cell Size
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