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Chromogranin A ELISA 试剂盒

This Pig Chromogranin A ELISA Kit is a Colorimetric ELISA Kit designed to quantify Pig Chromogranin A.
产品编号 ABIN6202858
发货至: 中国

Quick Overview for Chromogranin A ELISA 试剂盒 (ABIN6202858)

抗原

See all Chromogranin A (CHGA) ELISA试剂盒
Chromogranin A (CHGA)

适用

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Pig

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

62.5 pg/mL - 4000 pg/mL

应用范围

ELISA

样品类型

Cell Lysate, Plasma, Serum, Tissue Homogenate
  • 最低检测浓度

    62.5 pg/mL

    原理

    Pig Chromogranin A (CHGA) ELISA Kit

    Analytical Method

    Quantitative

    灵敏度

    37.5 pg/mL

    组件

    The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
    • Pre-coated 96-Well Microplate
    • Standard
    • Standard Diluent Buffer
    • Wash Buffer
    • Detection Reagent A
    • Detection Reagent B
    • Diluent A
    • Diluent B
    • TMB Substrate
    • Stop Solution
    • Plate Sealer

    试剂未包括

    • 37 °C incubator
    • Multi and single channel pipettes and sterile pipette tips
    • Squirt bottle or automated microplate washer
    • 1.5 mL tubes
    • Distilled water
    • Absorbent filter papers
    • 100 mL and 1 liter graduated cylinders
    • Microplate reader (wavelength: 450 nm)
    • ELISA Shaker
  • 应用备注

    Optimal dilutions/concentrations should be determined by the end user.

    说明

    The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

    板类型

    Pre-coated

    实验精密度

    Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Chromogranin A (CHGA) were tested 20 times on one plate, respectively.

    Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Chromogranin A (CHGA) were tested on 3 different plates, 8 replicates in each plate.

    CV (%) = (Standard Deviation / mean) x 100

    Intra-Assay: CV<10%

    Inter-Assay: CV<10%

    限制

    仅限研究用
  • 储存条件

    4 °C

    储存方法

    Shipped at 4 °C. Upon receipt, store the kit according to the storage instruction in the kit's manual.

    有效期

    6 months
  • 抗原 See all Chromogranin A (CHGA) ELISA试剂盒

    Chromogranin A (CHGA)

    别名

    Chromogranin A

    背景

    CMGA_PIG

    基因ID

    397540

    UniProt

    P04404

    途径

    Negative Regulation of Hormone Secretion, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling
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