Abeta 1-42 ELISA 试剂盒
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Quick Overview for Abeta 1-42 ELISA 试剂盒 (ABIN6200368)
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检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 12.35 pg/mL
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原理
- This kit is based on competitive enzyme-linked immuno-sorbent assay technology.
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Analytical Method
- Quantitative
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灵敏度
- < 6.2 pg/mL
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组件
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The kit components listed are for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- Pre-coated 96-Well Microplate
- Standard
- Standard Diluent Buffer
- Wash Buffer
- Detection Reagent A
- Detection Reagent B
- Diluent A
- Diluent B
- TMB Substrate
- Stop Solution
- Plate Sealer
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试剂未包括
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- 37 °C incubator
- Multi and single channel pipettes and sterile pipette tips
- Squirt bottle or automated microplate washer
- 1.5 mL tubes
- Distilled water
- Absorbent filter papers
- 100 mL and 1 liter graduated cylinders
- Microplate reader (wavelength: 450 nm)
- ELISA Shaker
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Amyloid beta 1-42 (Abeta 1-42) ELISA Kit
Abeta 1-42 适用: 人 Colorimetric Sandwich ELISA 4.688 pg/mL - 300 pg/mL Plasma, Serum, Tissue Homogenate
Amyloid beta 1-42 (Abeta 1-42) ELISA KitAbeta 1-42 适用: 大鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Amyloid beta 1-42 (Abeta 1-42) ELISA KitAbeta 1-42 适用: 人 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cerebrospinal Fluid, Plasma, Serum, Tissue Homogenate
Amyloid beta 1-42 (Abeta 1-42) ELISA KitAbeta 1-42 适用: 猴 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
Amyloid beta 1-42 (Abeta 1-42) ELISA KitAbeta 1-42 适用: 大鼠 Colorimetric Sandwich ELISA 0.9 pg/mL - 60 pg/mL Plasma, Serum, Tissue Homogenate
Amyloid beta 1-42 (Abeta 1-42) ELISA KitAbeta 1-42 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Amyloid beta 1-42 (Abeta 1-42) ELISA KitAbeta 1-42 适用: 人 Colorimetric Competition ELISA 3.7 pg/mL - 300 pg/mL Plasma, Serum
Amyloid beta 1-42 (Abeta 1-42) ELISA KitAbeta 1-42 适用: 小鼠 Colorimetric Competition ELISA 6.17 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Amyloid beta 1-42 (Abeta 1-42) ELISA KitAbeta 1-42 适用: 大鼠 Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
Amyloid beta 1-42 (Abeta 1-42) ELISA KitAbeta 1-42 适用: 小鼠 Colorimetric Sandwich ELISA 3.13 pg/mL - 200 pg/mL Cell Culture Supernatant, Plasma, Serum
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样本量
- 50 µL
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板类型
- Pre-coated
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实验流程
- This kit is based on competitive enzyme-linked immuno-sorbent assay technology. An antibody is pre-coated onto a 96-well plate. Standards, test samples, and biotin-conjugated reagent are added to the wells and incubated. A competitive inhibition reaction takes place between the biotin-labelled Abeta42 and the unlabelled- Abeta42 on the pre-coated antibody. The HRP-conjugated reagent is then added, and the whole plate is incubated. Unbound conjugates are removed using wash buffer at each stage. TMB substrate is used to quantify the HRP enzymatic reaction. After TMB substrate is added, only wells that contain sufficient Abeta42 will produce a blue coloured product, which then changes to yellow after adding the acidic stop solution. The intensity of the color yellow is inversely proportional to the Abeta42 amount bound on the plate. The OD is measured spectrophotometrically at 450 nm in a microplate reader, from which the concentration of Abeta42 can be calculated.
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试剂准备
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This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- 1) Standard: Prepare the standard with the recommended volume of Standard Diluent Buffer, to make the standard solution. Then use the Standard Diluent buffer to carry out serial dilutions of the standard solution, as instructed in the Protocol.
- 2) Wash Buffer: Dilute the concentrated Wash Buffer with distilled water, as instructed in the Protocol.
- 3) Detection Reagent Preparation: Calculate the total volume of working solution required. Dilute Detection Reagent A and Detection Reagent B with Diluent A and Diluent B, respectively, at 1:100.
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实验流程
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This procedure is provided for reference only. The product manual may differ slightly. The product should be used as stated on the product manual included and delivered together with the product.
- 1) Set standard, test samples and control wells.
- 2) Aliquot 50 μL of diluted standard into the standard wells.
- 3) Aliquot 50 μL of Standard Diluent buffer into the control (zero) well.
- 4) Aliquot 50 μL of diluted samples into the sample wells.
- 5) Immediately aliquot 50 μL of Detection Reagent A to each well. Incubate for 1 hr at 37 °C.
- 6) Wash 3 times.
- 7) Aliquot 100 μL of Detection Reagent B to each well. Incubate for 30 mins at 37 °C.
- 8) Wash 5 times.
- 9) Aliquot 90 μL of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C.
- 10) Aliquot 50 μL of Stop Solution.
- 11) Measure the OD at 450 nm.
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结果分析
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This assay is competitive, therefore there is an inverse correlation between Abeta42 concentration in the sample and the absorbance measured. Create a graph with the log of the standard concentration (y-axis) and average absorbance measured (x-axis). Apply a best fit trendline through the standard points. The Abeta42 concentration of the samples can be interpolated from the standard curve.
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实验精密度
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Intra-assay Precision (Precision within an assay): 3 samples with low, medium and high levels of Amyloid beta 42 (Abeta42) were were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, medium and high levels of Amyloid beta 42 (Abeta42) were tested on 3 different plates, 8 replicates in each plate.
CV (%) = (Standard Deviation / mean) x 100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Shipped at 4°C. Upon receipt, store the kit according to the storage instruction in the kit's manual.The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5% within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.
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有效期
- 6 months
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- Abeta 1-42 (Amyloid beta 1-42 (Abeta 1-42))
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别名
- Amyloid Beta 42
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