FMN (Flavin Mononucleotide) ELISA 试剂盒

• Pre-coated 96-Well Microplate
• Standard
• Standard Diluent Buffer
• Wash Buffer
• Detection Reagent A
• Detection Reagent B
• Diluent A
• Diluent B
• TMB Substrate
• Stop Solution
• Plate Sealer

• 37°C incubator
• Multi and single channel pipettes and sterile pipette tips
• Squirt bottle or automated microplate washer
• 1.5 ml tubes
• Distilled water
• Absorbent filter papers
• 100 ml and 1 liter graduated cylinders
• Microplate reader (wavelength: 450 nm)
• ELISA Shaker

Stability: The stability of the kit is determined by the rate of activity loss. The loss rate is less than 5 % within the expiration date under appropriate storage conditions. To minimize performance fluctuations, operation procedures and lab conditions should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same user throughout.

Recommended dilutions: Optimal dilutions/concentrations should be determined by the end user.

1. Set standard, test samples and control wells.
2. Aliquot 50 μl of diluted standard into the standard wells.
3. Aliquot 50 μl of Standard Diluent buffer into the control (zero) well.
4. Aliquot 50 μl of diluted samples into the sample wells.
5. Immediately aliquot 50 μl of Detection Reagent A to each well. Incubate for 45 mins at 37 °C.
6. Wash 3 times.
7. Aliquot 100 μl of Detection Reagent B to each well. Incubate for 30 mins at 37 °C.
8. Wash 5 times.
9. Aliquot 90 μl of TMB Substrate to each well. Incubate for 10-20 mins at 37 °C.
10. Aliquot 50 μl of Stop Solution.
11. Measure the OD at 450 nm.