alpha Fetoprotein ELISA 试剂盒
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Quick Overview for alpha Fetoprotein ELISA 试剂盒 (ABIN577112)
抗原
See all alpha Fetoprotein (AFP) ELISA试剂盒适用
检测方法
实验类型
应用范围
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原理
- This AFP enzyme-linked immunosorbent assay (ELISA) applies a technique called quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for AFP. Standards or samples are then added to the appropriate microtiter plate wells and incubated. AFP if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove any unbound AFP and other components of sample. In order to quantitate the amount of AFP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody specific for AFP is added to each well to
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Analytical Method
- Quantitative
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灵敏度
- The minimum detectable dose of AFP was determined by adding two standard deviations to the mean optical density value of 16 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose of human AFP using a standard curve generated is 2.0 ng/mL .
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组件
- Standards: 1 set/2 vials
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存液
- Without preservative
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- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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别名
- Alpha Fetoprotein (AFP)
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途径
- C21-Steroid Hormone Metabolic Process
抗原 See all alpha Fetoprotein (AFP) ELISA试剂盒
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