Interleukin 17a ELISA 试剂盒
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Quick Overview for Interleukin 17a ELISA 试剂盒 (ABIN577108)
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See all Interleukin 17a (IL17A) ELISA试剂盒适用
检测方法
实验类型
应用范围
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原理
- This IL-17A enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been S7.5(01) IL-17A 2 _x000C_ pre-coated with a monoclonal antibody specific to IL-17A. Standards or samples are then added to the appropriate microtiter plate wells. A biotin-conjugated antibody preparation specific for IL-17A was added and incubated. IL-17A, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound IL-17A and other components of the sample. Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. The wells are thoroughly washed to remove all unbound HRP-conjugated Avidin, and a TMB (3,3'5, 5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-17A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm 2 nm. In order to measure the concentration of IL-17A in the samples this kit includes two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant testing.) According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D) versus IL-17A concentration (pg/mL). The concentration of IL-17A in the samples is then determined by comparing the O.D. of the samples to the standard curve. LIMITATIONS OF THE PROCEDURE FOR LABORATORY RESEARCH USE ONLY, NOT FOR USE IN DIAGNOSTIC PROCEDURES. As manufacturers we take great care to ensure that our products are suitable for use with all validated sample types, as designated in the product insert. However, it is possible that in some cases, high levels of interfering factors may cause unusual results. The kit should not be used beyond the expiration date on the kit label. It is important that the Calibrator Diluent selected for the standard curve be consistent with the samples being assayed. If samples generate values higher than the highest standard, dilute the samples with the appropriate Calibrator Diluent and repeat the assay. Any variation in standard diluent, operator, pipetting technique, washing technique, incubation time or temperature, and kit age can cause variation in binding. Soluble receptors or other binding proteins present in biological samples do not necessarily interfere with the measurement of ligands in samples. However, until the factors have been tested, the possibility of interference cannot be excluded. S7.5(01) IL-17A 3
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Analytical Method
- Quantitative
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灵敏度
- The minimum detectable dose of IL-17A was determined by adding two standard deviations to the mean optical density value of 20 zero standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose of human IL-17A calculated from calibrate I diluted standard curve was <5 pg/mL .
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组件
- Standards: 1 set/2 vials
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Interleukin 17A (IL17A) ELISA Kit
IL17A 适用: 人 Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Cell Culture Supernatant, Serum, Tissue Homogenate, Urine
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 大鼠 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 小鼠 AA 22-158 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 人 AA 20-155 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 小鼠 Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 人 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 小鼠 Colorimetric Sandwich ELISA 6-600 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 大鼠 Colorimetric Sandwich ELISA 3.13-200 pg/mL Cell Culture Supernatant, Plasma, Serum
Interleukin 17A (IL17A) ELISA KitIL17A 适用: 小鼠 Colorimetric Sandwich ELISA 15.63-1000 pg/mL Cell Culture Supernatant, Plasma, Serum
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存液
- Without preservative
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- Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
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别名
- Interleukin-17 A (IL-17A)
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背景
- Interleukin-15 (IL-15), a modest 14-15 kDa glycoprotein, is produced by monocytes and dendritic cells and at maturity reaches 114 amino acid residues in length. IL-15 is a member of the four helix bound cytokine family that includes IL-2, IL-3, IL-5, IL-6, IL-7 and IL-9. Although the structure of IL-15 has not been determined, it is predicted to be similar to IL-2 and other members of the four-helix bundle cytokine family. IL-15 mRNA has been discovered in the placenta, skeletal muscle, kidneys, lungs, heart, fibroblasts, epithelial cells, dendritic cells, and monocytes. Human IL-15 shares approximately 97% and 73% amino acid sequence identity with simian and mouse IL-15, respectively. Both human and simian IL-15 molecules are active with mouse cells. The biological activities of IL-15 are comparable to IL-2: IL-15 induces the proliferation of activated CD4-CD8-, CD4+CD8+, CD4+, and CD8+ cells and dendritic epidermal T cells. IL-15 co-stimulates proliferation of B cells activated with immobilized anti-human IgM or phorbol ester, but has no stimulatory effect on resting B cells. In combination with recombinant CD4L, IL-15 is a potent inducer of polyclonal IgM, IgG1, and IgA secretion, but does not cause production of IgG4 or IgE. The development, survival, and activation of natural killer (NK) cells is related to IL-15. IL-15 also affects the non-lymphoid tissues, such as muscle, brain, microglia, and mast cells. Abnormal levels of IL-15 have been reported in rheumatoid arthritis, inflammatory bowel disease, and in diseases associated with the retroviruses HIV and HTLV-I. This IL-15 ELISA is a ready-to-use 4.5-hour solid phase immunoassay readily capable of measuring IL-15 levels in serum, plasma, cell culture supernatant, and other biological fluids in the range of to 2 pg/mL. This assay has shown no cross-reactivity with other cytokines tested, and is expected to be used effectively for further investigations into the relationship between IL-15 and the various conditions mentioned. S7.5(2) IL-15 2
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