CCL7 ELISA 试剂盒
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Quick Overview for CCL7 ELISA 试剂盒 (ABIN577078)
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See all CCL7 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
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最低检测浓度
- 30 pg/mL
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原理
- This MCP-3 enzyme linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for MCP-3. Standards or samples are then added - the appropriate microtiter plate wells and incubated. MCP-3 if present, will bind and become immobilised by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed - remove unbound MCP-3 and other components of sample. In order - quantitate the amount of MCP-3 present in the sample, a standardised preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody specific for MCP-3 is added - each well to
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Analytical Method
- Quantitative
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灵敏度
- < 30 pg/mL
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组件
- Standards: 1 set/2 vials
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Chemokine (C-C Motif) Ligand 7 (CCL7) ELISA Kit
CCL7 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 7 (CCL7) ELISA KitCCL7 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 7 (CCL7) ELISA KitCCL7 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 7 (CCL7) ELISA KitSmall Sample CCL7 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 7 (CCL7) ELISA KitCCL7 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 7 (CCL7) ELISA KitCCL7 适用: 大鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 7 (CCL7) ELISA KitCCL7 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Chemokine (C-C Motif) Ligand 7 (CCL7) ELISA KitCCL7 适用: 人 AA 24-99 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 7 (CCL7) ELISA KitCCL7 适用: 小鼠 AA 24-97 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Chemokine (C-C Motif) Ligand 7 (CCL7) ELISA KitCCL7 适用: 大鼠 AA 24-97 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
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板类型
- Pre-coated
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限制
- 仅限研究用
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储存液
- Without preservative
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- CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))
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别名
- Monocyte Chemotactic Protein-3 (MCP-3)
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背景
- The determination of serum or plasma levels of thyroid-stimulating hormone (TSH, or thyrotropin) is recognized as a sensitive method in the diagnosis of primary and secondary hypothyroidism. Thyroid-stimulating hormone is secreted by the anterior lobe of the pituitary gland and induces the production and release of thyroxine and triiodothyronine from the thyroid gland. It is glycoprotein with a molecular weight of approximately 28, Dalton, consisting of two chemically different subunits, alpha and beta. Although the concentration of TSH in the blood is extremely low, it is essential for the maintenance of normal thyroid function. The release of TSH is regulated by a TSH- releasing hormone (TRH) produced by the hypothalamus. The levels of TSH and TRH are inversely related to the level of thyroid hormone. When there is a high level of thyroid hormone in the blood, less TRH is released by the hypothalamus, so less TSH is secreted by the pituitary. The opposite action will occur when there is decreased thyroid hormone in the blood. This process is known as a negative feedback mechanism and is responsible for maintaining the proper blood levels of these hormones. Conventional TSH assays are generally accepted as an important tool in the diagnosis of primary and secondary hypothyroidism, but they offer limited clinical utility in the assessment of hyperthyroidism due to lack of sensitivity. This Ultra-sensitive TSH assay offers a sensitivity of .5 IU/mL and hence allows discrimination between hyperthyroid and normal patient populations. TSH and the pituitary glycoproteins: luteinizing hormone (LH), follicle-stimulating hormone (FSH), and human chorionic gonadotropin (hCG) have identical alpha chains. The beta chain is distinct but does contain identical amino acid sequences, which can cause considerable cross-reactivity with some polyclonal TSH antisera. The use of monoclonal antibodies in this Ultra-sensitive Human TSH ELISA Kit eliminates this interference, which could result in falsely elevated TSH values in either menopausal or pregnant females, a population whose evaluation of thyroid status is clinically significant. S7.5(2) U-TSH 2
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