Total Bile Acid (TBA) Colorimetric Assay Kit
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北京 101111
Quick Overview for Total Bile Acid (TBA) Colorimetric Assay Kit (ABIN7121144)
抗原
检测方法
应用范围
样品类型
Analytical Method
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检测范围
- 1.77 μmol/L - 40 μmol/L
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最低检测浓度
- 1.77 μmol/L
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原理
- The Total Bile Acid (TBA) Assay Kit is biochemical kit in Colorimetric method,can be used for in vitro quantitative determination of total bile acids content in serum.
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灵敏度
- 1.36 μmol/L
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产品特性
- With S-NAD+ as hydrogen receptor, 3α-hydroxy steroid dehydrogenase catalyzed the dehydrogenation of bile acids to produce 3-ketone steroids, transforming S-NAD+ into S-NADH. Meanwhile, NADH was used as hydrogen donor. 3α-hydroxy steroid dehydrogenase catalyzed the production of bile acids from 3-ketone steroids. Through the enzyme cycle reaction, S-NADH is continuously generated, which has the maximum absorption peak at 405 nm. Measure the OD value at 405 nm and the changes of absorbance is proportional to the concentration of bile acid.
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试剂未包括
- Spectrophotometry (Visible range), Biochemistry analyzer
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实验时间
- 25 min
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实验精密度
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Inter-assay precision: 3.7%
Intra-assay precision: 3.4%
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限制
- 仅限研究用
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注意事项
- Protect from light.
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储存条件
- 4 °C
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储存方法
- This product can be stored at 2~8°C for 12 months with shading light.
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有效期
- 12 months
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- Total Bile Acide
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别名
- Total Bile Acid
抗原
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