H+K+-ATPase Activity Assay Kit
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北京 101111
Quick Overview for H+K+-ATPase Activity Assay Kit (ABIN7121129)
抗原
See all ATP4b 试剂盒检测方法
应用范围
样品类型
Analytical Method
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原理
- The H+/K+-ATPase Assay Kit is biochemical kit in Colorimetric method
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产品特性
- ATPase can decompose ATP to produce ADP and inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. The inorganic phosphorus reacts with ammonium molybdate in acidic solution to form ammonium molybdate compound, which is reduced with reducing agent to form molybdenum blue, and has absorption peak at 660 nm. Determine the concentration of molybdenum blue to calculate the amount of inorganic phosphorus.
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试剂未包括
- Spectrophotometry (Visible range)
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实验时间
- 1 h
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实验精密度
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Inter-assay precision: 9.8%
Intra-assay precision: 4.4%
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限制
- 仅限研究用
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注意事项
- Protect from light.
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储存条件
- 4 °C,-20 °C
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储存方法
- This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
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有效期
- 12 months
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- ATP4b (ATPase, H+/K+ Exchanging, beta Polypeptide (ATP4b))
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别名
- H+K+-ATPase
抗原
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