MYOD1 ELISA 试剂盒
Quick Overview for MYOD1 ELISA 试剂盒 (ABIN5706920)
抗原
See all MYOD1 ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 78.1 pg/mL
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Analytical Method
- Quantitative
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灵敏度
- 29 pg/mL
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Myogenic Differentiation 1 (MYOD1) ELISA Kit
MYOD1 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Myogenic Differentiation 1 (MYOD1) ELISA KitMYOD1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Myogenic Differentiation 1 (MYOD1) ELISA KitMYOD1 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual -
样本量
- 100 µL
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实验时间
- 1 - 4.5 h
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板类型
- Pre-coated
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实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately. -
限制
- 仅限研究用
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储存条件
- 4 °C
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有效期
- 12 months
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- MYOD1 (Myogenic Differentiation 1 (MYOD1))
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别名
- Myogenic Differentiation (MyoD)
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背景
- Alternative name: MYOD1, PUM, MYF3, bHLHc1, Myoblast Determination Protein 1, Myogenic Factor 3, Class C basic helix-loop-helix protein 1
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途径
- Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development
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