GRIN2B ELISA 试剂盒
Quick Overview for GRIN2B ELISA 试剂盒 (ABIN5706918)
抗原
See all GRIN2B ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.312 ng/mL
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Analytical Method
- Quantitative
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灵敏度
- 0.133 ng/mL
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Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) ELISA Kit
GRIN2B 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) ELISA KitGRIN2B 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) ELISA KitGRIN2B 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) ELISA KitGRIN2B 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B) ELISA KitGRIN2B 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual -
样本量
- 100 µL
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实验时间
- 1 - 4.5 h
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板类型
- Pre-coated
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实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately. -
限制
- 仅限研究用
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储存条件
- 4 °C
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有效期
- 12 months
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- GRIN2B (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 2B (GRIN2B))
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别名
- Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate 2B (GRIN2B)
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背景
- Alternative name: NMDAR2B, NR2B, hNR3, Glutamate [NMDA] receptor subunit epsilon-2, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3
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途径
- Response to Growth Hormone Stimulus, Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity
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