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Neuroligin 1 ELISA 试剂盒

This Colorimetric ELISA kit is designed for the quantitative measurement of 大鼠 Neuroligin 1.
产品编号 ABIN5706905
发货至: 中国
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Neuroligin 1 ELISA 试剂盒 (ABIN5706905)

抗原

See all Neuroligin 1 (NLGN1) ELISA试剂盒
Neuroligin 1 (NLGN1)

适用

大鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

78.1 pg/mL - 5000 pg/mL

应用范围

ELISA

样品类型

Plasma, Serum, Tissue Homogenate
  • 最低检测浓度

    78.1 pg/mL

    Analytical Method

    Quantitative

    灵敏度

    33 pg/mL
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    说明

    Pre-coated, ready to use 96-well strip plate,
    Standard (freeze dried),
    Standard Diluent,
    Detection Reagent A,
    Detection Reagent B,
    Assay Diluent A,
    Assay Diluent B,
    TMB substrate,
    Stop Solution,
    Wash Buffer(30 x concenthumane),
    Plate sealer for 96 wells,
    Instruction manual

    样本量

    100 µL

    实验时间

    1 - 4.5 h

    板类型

    Pre-coated

    实验流程

    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
    8. Add 50µL Stop Solution. Read at 450nm immediately.

    限制

    仅限研究用
  • 储存条件

    4 °C

    有效期

    12 months
  • 抗原 See all Neuroligin 1 (NLGN1) ELISA试剂盒

    Neuroligin 1 (NLGN1)

    背景

    Alternative name: Neuroligin I, Neuroligin-1

    途径

    Synaptic Membrane, Synaptic Vesicle Exocytosis
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