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Myosin X ELISA 试剂盒

This 人 Myosin X ELISA Kit specifically detects Myosin X in Plasma, Serum 和 Tissue Homogenate.
产品编号 ABIN5706904
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Myosin X ELISA 试剂盒 (ABIN5706904)

抗原

See all Myosin X (MYO10) products
Myosin X (MYO10)

适用

检测方法

Colorimetric

实验类型

Competition ELISA

检测范围

0.312 ng/mL - 20 ng/mL

应用范围

ELISA

样品类型

Plasma, Serum, Tissue Homogenate
  • 最低检测浓度

    0.312 ng/mL

    原理

    The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of MYO10 in human serum, plasma, tissue homogenates

    Analytical Method

    Quantitative

    灵敏度

    0.114 ng/mL
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    说明

    Pre-coated, ready to use 96-well strip plate,
    Standard (freeze dried),
    Standard Diluent,
    Detection Reagent A,
    Detection Reagent B,
    Assay Diluent A,
    Assay Diluent B,
    TMB substrate,
    Stop Solution,
    Wash Buffer(30 x concenthumane),
    Plate sealer for 96 wells,
    Instruction manual

    样本量

    50 µL

    实验时间

    1 - 4.5 h

    板类型

    Pre-coated

    实验流程

    1. Prepare all reagents, samples and standards
    2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C
    3. Aspirate and wash 3 times
    4. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
    5. Aspirate and wash 5 times
    6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
    7. Add 50µL Stop Solution. Read at 450 nm immediately.

    限制

    仅限研究用
  • 储存条件

    4 °C

    有效期

    12 months
  • 抗原 See all Myosin X (MYO10) products

    Myosin X (MYO10)

    背景

    Alternative name: Unconventional Myosin-X, Unconventional Myosin-10
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