Myosin X ELISA 试剂盒
This 人 Myosin X ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 Myosin X.
Quick Overview for Myosin X ELISA 试剂盒 (ABIN5706904)
抗原
See all Myosin X (MYO10) products
Myosin X (MYO10)
适用
人
检测方法
Colorimetric
实验类型
Competition ELISA
检测范围
0.312 ng/mL - 20 ng/mL
应用范围
ELISA
样品类型
Plasma, Serum, Tissue Homogenate
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最低检测浓度
- 0.312 ng/mL
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原理
- The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of MYO10 in human serum, plasma, tissue homogenates
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Analytical Method
- Quantitative
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灵敏度
- 0.114 ng/mL
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual -
样本量
- 50 µL
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实验时间
- 1 - 4.5 h
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板类型
- Pre-coated
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实验流程
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1. Prepare all reagents, samples and standards
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C
3. Aspirate and wash 3 times
4. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5. Aspirate and wash 5 times
6. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7. Add 50µL Stop Solution. Read at 450 nm immediately. -
限制
- 仅限研究用
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储存条件
- 4 °C
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有效期
- 12 months
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- Myosin X (MYO10)
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背景
- Alternative name: Unconventional Myosin-X, Unconventional Myosin-10
抗原 See all Myosin X (MYO10) products
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