OVA sIgG ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for OVA sIgG ELISA 试剂盒 (ABIN5706332)
抗原
See all OVA sIgG products适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 3.125 ng/mL
-
原理
- Rabbit OVA IgG (Ovalbumin IgG) ELISA Kit
-
Analytical Method
- Quantitative
-
特异性
- Specifically binds with OVA IgG , no obvious cross reaction with other analogues.
-
灵敏度
- 1.875 ng/mL
-
试剂未包括
-
- Microplate reader (wavelength: 450nm)
- 37 °C incubator (CO2 incubator for cell culture is not recommenced.)
- Automated plate washer or multi-channel pipette/5 mL pipettor
- Precision single (0.5-10μL
- 5-50μL
- 20-200μL
- 200-1000μL) and multi-channel pipette with disposable tips(Calibration is required before use.)
- Sterile tubes and Eppendorf tubes with disposable tips
- Absorbent paper and loading slot
- Deionized or distilled water
-
-
Ovalbumin Specific IgG (OVA sIgG) ELISA Kit
OVA sIgG 适用: 豚鼠 Colorimetric Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Ovalbumin Specific IgG (OVA sIgG) ELISA KitOVA sIgG 适用: 人 Colorimetric Indirect ELISA 0.313 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Ovalbumin Specific IgG (OVA sIgG) ELISA KitOVA sIgG 适用: 小鼠 Colorimetric Indirect ELISA 3.125 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
说明
-
Indirect ELISA
-
样本量
- 100 μL
-
实验时间
- 3 h
-
板类型
- Pre-coated
-
实验流程
- Step 1: Wash the plate twice before adding the standard and sample. Step 2: Add 100 µL standard or sample into each well, seal the plate and statically incubate for 90 minutes at 37 °C. Washing: Wash the plate three times and immerse for 1 min each time. Step 3: Add 100 µL HRP-antibody working solution, seal the plate and statically incubate for 30 minutes at 37 °C. Washing: Wash the plate five times and immerse for 1 min each time. Step 4: Add 90 µL TMB substrate solution, seal the plate and statically incubate for 10-20 minutes at 37 °C. (Accurate TMB visualization control is required.) Step 5: Add 50 µL stop solution. Read at 450nm immediately and calculate.
-
限制
- 仅限研究用
-
-
-
注意事项
- Avoid direct light exposure to antibody solutions
-
储存条件
- 4 °C
-
储存方法
- Store sealed Kit at 2-8° C. Do Not Cryopreserve. Store ELISA Microplate for 1 month at 2-8°C, Store for 12 month at -20°C. Put the leftover strips into a sealed foil bag with the desiccant
-
有效期
- 12 months
-
-
- OVA sIgG (Ovalbumin Specific IgG (OVA sIgG))
-
别名
- Ovalbumin IgG
-
物质类
- Antibody
-
背景
- OVA sIgG, Ovalbumin specific Immunoglobulin G
抗原 See all OVA sIgG products
-
