PLA2G7 ELISA 试剂盒
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Quick Overview for PLA2G7 ELISA 试剂盒 (ABIN5692232)
抗原
See all PLA2G7 (Lp-PLA2) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.78 ng/mL
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原理
- Sandwich Human PLA2G7/Lp-PLA2 ELISA Kit to quantitate Human PLA2G7 in cell culture supernatants, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: F22-N441Capture antibody and Detection antibody: monoclonal antibody from mouse, biotinylated polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 20 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Lipoprotein-Associated phospholipase A2 (Lp-PLA2) ELISA Kit
Lp-PLA2 适用: 人 Colorimetric Sandwich ELISA 0.62 ng/mL - 40 ng/mL Plasma, Serum
Lipoprotein-Associated phospholipase A2 (Lp-PLA2) ELISA KitLp-PLA2 适用: 人 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Lipoprotein-Associated phospholipase A2 (Lp-PLA2) ELISA KitLp-PLA2 适用: 小鼠 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Lipoprotein-Associated phospholipase A2 (Lp-PLA2) ELISA KitLp-PLA2 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Lipoprotein-Associated phospholipase A2 (Lp-PLA2) ELISA KitLp-PLA2 适用: 人 Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Lipoprotein-Associated phospholipase A2 (Lp-PLA2) ELISA KitLp-PLA2 适用: 小鼠 Colorimetric Sandwich ELISA 3.125 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Lipoprotein-Associated phospholipase A2 (Lp-PLA2) ELISA KitLp-PLA2 适用: 大鼠 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Plasma, Serum, Tissue Homogenate
Lipoprotein-Associated phospholipase A2 (Lp-PLA2) ELISA KitLp-PLA2 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Plasma, Serum
Lipoprotein-Associated phospholipase A2 (Lp-PLA2) ELISA KitLp-PLA2 适用: 小鼠 Colorimetric Sandwich ELISA 7.81-500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Lipoprotein-Associated phospholipase A2 (Lp-PLA2) ELISA KitLp-PLA2 适用: 人 Colorimetric Sandwich ELISA 1.56-100 ng/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4.7%, 5.5%, 4.9%
Inter-Assay Precision (CV%): 6.1%, 6.6%, 6.7% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- PLA2G7 (Lp-PLA2) (Lipoprotein-Associated phospholipase A2 (Lp-PLA2))
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别名
- PLA2G7
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背景
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Background: Lipoprotein-associated phospholipase A2 (Lp-PLA2) also known as platelet-activating factor acetylhydrolase (PAF-AH) is a phospholipase A2 enzyme that in humans is encoded by the PLA2G7 gene. Lp-PLA2 is a 45- kDa protein of 441 amino acids. It is one of several PAF acetylhydrolases. The protein encoded by this gene is a secreted enzyme that catalyzes the degradation of platelet-activating factor to biologically inactive products. Defects in this gene are a cause of platelet-activating factor acetylhydrolase deficiency. Two transcript variants encoding the same protein have been found for this gene.
Gene Full Name: phospholipase A2 group VII
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基因ID
- 7941
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UniProt
- Q13093
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途径
- Peptide Hormone Metabolism
抗原 See all PLA2G7 (Lp-PLA2) ELISA试剂盒
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