CXCL7 ELISA 试剂盒
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Quick Overview for CXCL7 ELISA 试剂盒 (ABIN5692229)
抗原
See all CXCL7 (PPBP) ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 15.6 pg/mL
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原理
- Sandwich Mouse PPBP/CXCL7 ELISA Kit to quantitate Mouse Ppbp in cell culture supernatants, serum and plasma (heparin, EDTA).
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: K40-Y113Capture antibody and Detection antibody: monoclonal antibody from rat, biotinylated polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 2 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP) ELISA Kit
PPBP 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP) ELISA KitPPBP 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP) ELISA KitPPBP 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP) ELISA KitPPBP 适用: 人 Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Plasma, Serum, Tissue Homogenate
Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP) ELISA KitPPBP 适用: 小鼠 Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP) ELISA KitPPBP 适用: 大鼠 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP) ELISA KitPPBP 适用: 兔 Colorimetric Sandwich ELISA 0.24 ng/mL - 15 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP) ELISA KitQuick PPBP 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Biological Fluids, Plasma, Serum
Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP) ELISA KitQuick PPBP 适用: 大鼠 Colorimetric Sandwich ELISA 15.63 pg/mL - 1000 pg/mL Biological Fluids, Plasma, Serum
Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP) ELISA KitPPBP 适用: 人 AA 59-128 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 5.3%, 6.8%, 4.1%
Inter-Assay Precision (CV%): 6.6%, 7%, 4.2% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- CXCL7 (PPBP) (Pro-Platelet Basic Protein (Chemokine (C-X-C Motif) Ligand 7) (PPBP))
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别名
- Ppbp
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背景
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Background: Chemokine (C-X-C motif) ligand 7 (CXCL7), also called PPBP or SCYB7, is a human gene. The encoded protein, Chemokine (C-X-C motif) ligand is a small cytokine belonging to the CXC chemokine family. CXCL7 is mapped to 4q13.3. It is a protein that is released in large amounts from platelets following their activation. CXCL7 is the precursor of the 2 platelet alpha-granule proteins, platelet basic protein (PBP) and connective tissue-activating peptide III (CTAP3). It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator. It also stimulates the formation and secretion of plasminogen activator by synovial cells.
Gene Full Name: pro-platelet basic protein
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基因ID
- 57349
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UniProt
- Q9EQI5
抗原 See all CXCL7 (PPBP) ELISA试剂盒
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