ENPP2 ELISA 试剂盒
Our Local Distributor
北京 101111
Quick Overview for ENPP2 ELISA 试剂盒 (ABIN5692213)
抗原
See all ENPP2 ELISA试剂盒抗原表位
适用
检测方法
实验类型
检测范围
应用范围
样品类型
-
-
最低检测浓度
- 625 pg/mL
-
原理
- Sandwich Human Autotaxin ELISA Kit to quantitate Human ENPP2 in cell culture supernatants, serum, plasma (heparin) , urine and human milk.
-
Analytical Method
- Quantitative
-
特异性
-
Expression system for standard: NS0
Immunogen sequence: A36-I863Capture antibody and Detection antibody: a polyclonal antibody from goat, a biotinylated polyclonal antibody from goat
-
交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
-
灵敏度
- < 10 pg/mL
-
组件
-
- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
-
试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
-
-
Ectonucleotide Pyrophosphatase / phosphodiesterase 2 (ENPP2) ELISA Kit
ENPP2 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Plasma, Serum, Tissue Homogenate
Ectonucleotide Pyrophosphatase / phosphodiesterase 2 (ENPP2) ELISA KitENPP2 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Ectonucleotide Pyrophosphatase / phosphodiesterase 2 (ENPP2) ELISA KitENPP2 适用: 人 Colorimetric Sandwich ELISA 93.75 pg/mL - 6000 pg/mL Plasma, Serum, Tissue Homogenate
Ectonucleotide Pyrophosphatase / phosphodiesterase 2 (ENPP2) ELISA KitENPP2 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Ectonucleotide Pyrophosphatase / phosphodiesterase 2 (ENPP2) ELISA KitENPP2 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
-
-
应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
-
样本量
- 100 μL
-
板类型
- Pre-coated
-
实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
-
实验精密度
-
Intra-Assay Precision (CV%): 6.9%, 6.5%, 5.5%
Inter-Assay Precision (CV%): 7%, 6.6%, 6.5% -
限制
- 仅限研究用
-
-
-
注意事项
- Avoid multiple freeze-thaw cycles.
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
-
有效期
- 12 months
-
-
- ENPP2 (Ectonucleotide Pyrophosphatase / phosphodiesterase 2 (ENPP2))
-
别名
- ENPP2
-
背景
-
Background: Autotaxin, also known as ectonucleotide pyrophosphatase/phosphodiesterase family member 2 (E-NPP 2), is an enzyme that in humans is encoded by the ENPP2 gene. The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified.
Gene Full Name: ectonucleotide pyrophosphatase/phosphodiesterase 2
-
基因ID
- 5168
-
UniProt
- Q13822
抗原 See all ENPP2 ELISA试剂盒
-
