SCGB1A1 ELISA 试剂盒
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Quick Overview for SCGB1A1 ELISA 试剂盒 (ABIN5692211)
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适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 156 pg/mL
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原理
- Sandwich Rat SCGB1A1/uteroglobin ELISA Kit to quantitate Rat Scgb1a1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
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Analytical Method
- Quantitative
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特异性
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Expression system for standard: E.coli
Immunogen sequence: S20-V96Capture antibody and Detection antibody: a monoclonal antibody from mouse, a biotinylated polyclonal antibody from goat
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交叉反应 （详细）
- There is no detectable cross-reactivity with other relevant proteins.
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灵敏度
- < 10 pg/mL
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组件
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- Pre-coated 96-well strip microplate
- Standard
- Biotinylated antibody (100x)
- Avidin-Biotin-Peroxidase Complex (100x)
- Sample Diluent
- Antibody Diluent
- Avidin-Biotin-Peroxidase Diluent
- Color Developing Reagent (TMB)
- Stop Solution
- Wash Buffer (25x)
- Adhesive plate sealers
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试剂未包括
- Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA Kit
SCGB1A1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Lung Lavage Fluid, Plasma, Serum, Tissue Homogenate
Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA KitSCGB1A1 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Lung Lavage Fluid, Plasma, Serum, Tissue Homogenate
Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA KitSCGB1A1 适用: 人 Colorimetric Sandwich ELISA 0.313 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA KitSCGB1A1 适用: 小鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA KitSCGB1A1 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Lung Lavage Fluid, Plasma, Serum, Tissue Homogenate
Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA KitSmall Sample SCGB1A1 适用: 小鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Lung Lavage Fluid, Plasma, Serum, Tissue Homogenate
Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA KitSCGB1A1 适用: 人 AA 22-91 Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Milk, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Urine
Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA KitSCGB1A1 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Lung Lavage Fluid, Plasma, Serum, Tissue Homogenate
Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA KitSCGB1A1 适用: 大鼠 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Lung Lavage Fluid, Plasma, Serum, Tissue Homogenate
Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1) ELISA KitSCGB1A1 适用: 大鼠 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Lung Lavage Fluid, Plasma, Serum, Tissue Homogenate
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应用备注
- Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
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样本量
- 100 μL
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板类型
- Pre-coated
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实验流程
- ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.
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实验精密度
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Intra-Assay Precision (CV%): 4%, 7.7%, 6.6%
Inter-Assay Precision (CV%): 4.3%, 8.6%, 7.8% -
限制
- 仅限研究用
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注意事项
- Avoid multiple freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
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有效期
- 12 months
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- SCGB1A1 (Secretoglobin, Family 1A, Member 1 (Uteroglobin) (SCGB1A1))
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别名
- Scgb1a1
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背景
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Background: SCGB1A1 (secretoglobin family 1A member 1) also known as Uteroglobin is a protein that in humans is encoded by the SCGB1A1 gene. SCGB1A1 is the founding member of the secretoglobin family of small, secreted, disulfide-bridged dimeric proteins found only in mammals. This protein is specifically expressed in Clara cells in the lungs. Numerous studies demonstrated that UG is a multifunctional protein with antiinflammatory/ immunomodulatory properties. It inhibits soluble phospholipase A (2) activity and binds and perhaps sequesters hydrophobic ligands such as progesterone, retinols, polychlorinated biphenyls, phospholipids, and prostaglandins. In addition to its antiinflammatory activities, UG manifests antichemotactic, antiallergic, antitumorigenic, and embryonic growth-stimulatory activities.
Gene Full Name: secretoglobin family 1A member 1
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基因ID
- 25575
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UniProt
- P17559
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