HYAL1 ELISA 试剂盒
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- 抗原 See all HYAL1 ELISA试剂盒
- HYAL1 (Hyaluronidase-1 (HYAL1))
- 抗原表位
- AA 22-435
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 15.6 pg/mL - 1000 pg/mL
- 最低检测浓度
- 15.6 pg/mL
- 应用范围
- ELISA
- 原理
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Hyaluronidase1/HYAL1. 96wells/kit, with removable strips.
- 品牌
- PicoKine™
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma (EDTA - heparin), Serum
- Analytical Method
- Quantitative
- 特异性
- Expression system for standard: NSO, Immunogen sequence: F22-W435
- 灵敏度
- < 10 pg/mL
- 组件
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96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
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Hyaluronidase-1 (HYAL1) ELISA Kit
HYAL1 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Hyaluronidase-1 (HYAL1) ELISA KitHYAL1 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Hyaluronidase-1 (HYAL1) ELISA KitHYAL1 适用: 大鼠 Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Tissue Homogenate
Hyaluronidase-1 (HYAL1) ELISA KitHYAL1 适用: Cow Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Tissue Homogenate
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- 实验时间
- 15 min
- 板类型
- Pre-coated
- 限制
- 仅限研究用
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- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
- 有效期
- 12 months
-
- 抗原 See all HYAL1 ELISA试剂盒
- HYAL1 (Hyaluronidase-1 (HYAL1))
- 别名
- HYAL1 (HYAL1 产品)
- 别名
- HYAL-1 ELISA Kit, LUCA1 ELISA Kit, NAT6 ELISA Kit, Hya1 ELISA Kit, Hyal-1 ELISA Kit, hyaluronoglucosaminidase 1 ELISA Kit, HYAL1 ELISA Kit, Hyal1 ELISA Kit
- 背景
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Synonyms: Hyaluronidase-1, Hyal-1, Hyaluronoglucosaminidase-1, Lung carcinoma protein 1, LuCa-1, HYAL1, LUCA1
Tissue Specificity: Highly expressed in the liver, kidney and heart. Weakly expressed in lung, placenta and skeletal muscle. No expression detected in adult brain. Isoform 1 is expressed only in bladder and prostate cancer cells, G2/G3 bladder tumor tissues and lymph node specimens showing tumor invasive tumors cells. Isoform 3, isoform 4, isoform 5 and isoform 6 are expressed in normal bladder and bladder tumor tissues.
Background: Hyaluronidase-1, also known as HYAL1 or LUCA1, is an enzyme that in humans is encoded by the HYAL1 gene. The gene is one of several related genes in a region of chromosome 3p21.3 associated with tumor suppression. This gene encodes a lysosomal hyaluronidase. Hyaluronidases intracellularly degrade hyaluronan, one of the major glycosaminoglycans of the extracellular matrix. Hyaluronan is thought to be involved in cell proliferation, migration and differentiation. This enzyme is active at an acidic pH and is the major hyaluronidase in plasma. Mutations in this gene are associated with mucopolysaccharidosis type IX, or hyaluronidase deficiency.
Cellular Localisation: Secreted - UniProt
- Q12794
- 途径
- Glycosaminoglycan Metabolic Process
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