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SMPD3 ELISA 试剂盒
This Human SMPD3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human SMPD3 in Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate.
Quick Overview for SMPD3 ELISA 试剂盒 (ABIN5690953)
抗原
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SMPD3
(Sphingomyelin Phosphodiesterase 3 (SMPD3))
适用
All reactivities for SMPD3 ELISA试剂盒
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
样品类型
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
SMPD3ELISA Kit详情
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原理
Human SMPD3 ELISA Kit.
Analytical Method
Quantitative
特异性
This ELISA antibody pair recognizes Human SMPD3.
产品特性
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
组件
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
试剂未包括
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternatives
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使用细节
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板类型
Pre-coated
实验流程
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
限制
仅限研究用
贮存及处理
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储存条件
4 °C
有效期
6 months
SMPD3目标详情
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SMPD3
(Sphingomyelin Phosphodiesterase 3 (SMPD3))
别名
SMPD3
基因ID
55512
UniProt
Q9NY59
途径
Hormone Transport
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