s100a4 ELISA 试剂盒
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- 抗原 See all s100a4 (S100A4) ELISA试剂盒
- s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human S100A4 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human S100A4.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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S100 Calcium Binding Protein A4 (S100A4) ELISA Kit
S100A4 适用: 人, 小鼠, 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
S100 Calcium Binding Protein A4 (S100A4) ELISA KitHigh Sensitivity S100A4 适用: 人 Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 6 months
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- 抗原 See all s100a4 (S100A4) ELISA试剂盒
- s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))
- 别名
- S100A4 (S100A4 产品)
- 别名
- 18A2 ELISA Kit, 42A ELISA Kit, CAPL ELISA Kit, FSP1 ELISA Kit, MTS1 ELISA Kit, P9KA ELISA Kit, PEL98 ELISA Kit, 42a ELISA Kit, Capl ELISA Kit, FSp1 ELISA Kit, Mts1 ELISA Kit, PeL98 ELISA Kit, metastasin ELISA Kit, pk9a ELISA Kit, S100A4 ELISA Kit, P9ka ELISA Kit, RNP9KA ELISA Kit, S100 calcium binding protein A4 ELISA Kit, S100 calcium-binding protein A4 ELISA Kit, S100A4 ELISA Kit, S100a4 ELISA Kit
- 基因ID
- 6275
- UniProt
- P26447
- 途径
- S100 Proteins
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