PGPEP1 ELISA 试剂盒
This 人 PGPEP1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 PGPEP1 in Cell Culture Supernatant, Cell Samples, Plasma, Serum 和 Tissue Lysate.
Quick Overview for PGPEP1 ELISA 试剂盒 (ABIN5690932)
抗原
See all PGPEP1 products
PGPEP1
(Pyroglutamyl-Peptidase I (PGPEP1))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
应用范围
ELISA
样品类型
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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原理
- Human PGPEP-1 ELISA Kit.
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Analytical Method
- Quantitative
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特异性
- This ELISA antibody pair recognizes Human PGPEP-1.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- 4 °C
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有效期
- 6 months
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- PGPEP1 (Pyroglutamyl-Peptidase I (PGPEP1))
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别名
- PGPEP-1
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基因ID
- 54858
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UniProt
- Q9NXJ5
抗原 See all PGPEP1 products
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