NEDD8 ELISA 试剂盒
NEDD8
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all NEDD8 ELISA试剂盒
- NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human NEDD8 ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human NEDD8.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product NEDD8 ELISA Kit
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- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 6 months
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- 抗原 See all NEDD8 ELISA试剂盒
- NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
- 别名
- NEDD8 (NEDD8 产品)
- 别名
- NEDD-8 ELISA Kit, Rub1 ELISA Kit, CG10679 ELISA Kit, Dmel\\CG10679 ELISA Kit, NEDD8 ELISA Kit, dNEDD8 ELISA Kit, nedd8 ELISA Kit, ubiquitin ELISA Kit, si:zc14a17.5 ELISA Kit, GB17785 ELISA Kit, DDBDRAFT_0218116 ELISA Kit, DDBDRAFT_0238041 ELISA Kit, DDB_0218116 ELISA Kit, DDB_0238041 ELISA Kit, nedd8.S ELISA Kit, neural precursor cell expressed, developmentally down-regulated 8 ELISA Kit, neural precursor cell expressed, developmentally down-regulated gene 8 ELISA Kit, CG10679 gene product from transcript CG10679-RB ELISA Kit, Nedd8 ELISA Kit, ubiquitin-like protein Nedd8 ELISA Kit, neural precursor cell expressed, developmentally down-regulated 8 L homeolog ELISA Kit, NEDD8 ELISA Kit, Nedd8 ELISA Kit, nedd8 ELISA Kit, nedd8.L ELISA Kit
- 基因ID
- 4738
- UniProt
- Q15843
- 途径
- Ubiquitin Proteasome Pathway
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