LCK ELISA 试剂盒
LCK
适用: 人
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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- 抗原 See all LCK ELISA试剂盒
- LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 应用范围
- ELISA
- 原理
- Human LCK ELISA Kit.
- 样品类型
- Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
- Analytical Method
- Quantitative
- 特异性
- This ELISA antibody pair recognizes Human LCK.
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Top Product
- Discover our top product LCK ELISA Kit
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- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 6 months
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- 抗原 See all LCK ELISA试剂盒
- LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))
- 别名
- LCK (LCK 产品)
- 别名
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zgc:136695 ELISA Kit, LCK ELISA Kit, Hck-3 ELISA Kit, Lsk ELISA Kit, Lskt ELISA Kit, p56
ELISA Kit, p56Lck ELISA Kit, LSK ELISA Kit, YT16 ELISA Kit, p56lck ELISA Kit, pp58lck ELISA Kit, P56LCK ELISA Kit, tkl ELISA Kit, Lck1 ELISA Kit, Lcktkr ELISA Kit, LCK proto-oncogene, Src family tyrosine kinase ELISA Kit, lymphocyte protein tyrosine kinase ELISA Kit, lck ELISA Kit, LCK ELISA Kit, Lck ELISA Kit - 基因ID
- 3932
- UniProt
- P06239
- 途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling
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