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DIDO1 ELISA 试剂盒

DIDO1 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN5690865
发货至: 中国
  • 抗原 See all DIDO1 ELISA试剂盒
    DIDO1 (Death Inducer-Obliterator 1 (DIDO1))
    适用
    • 2
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human DIDO1/DIO-1 ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human DIDO1.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    6 months
  • 抗原 See all DIDO1 ELISA试剂盒
    DIDO1 (Death Inducer-Obliterator 1 (DIDO1))
    别名
    DIDO1 (DIO-1 (DIDO1 产品)
    别名
    5DI ELISA Kit, TXDI1 ELISA Kit, BYE1 ELISA Kit, C20orf158 ELISA Kit, DATF-1 ELISA Kit, DATF1 ELISA Kit, DIDO2 ELISA Kit, DIDO3 ELISA Kit, DIO-1 ELISA Kit, DIO1 ELISA Kit, dJ885L7.8 ELISA Kit, 6720461J16Rik ELISA Kit, C530043I07 ELISA Kit, D130048F08Rik ELISA Kit, Datf1 ELISA Kit, Dido2 ELISA Kit, Dido3 ELISA Kit, mKIAA0333 ELISA Kit, DIDO1 ELISA Kit, datf1l ELISA Kit, fi35b04 ELISA Kit, wu:fi35b04 ELISA Kit, zgc:158157 ELISA Kit, DKFZp469J1523 ELISA Kit, bye1 ELISA Kit, dio1 ELISA Kit, datf1 ELISA Kit, dido2 ELISA Kit, dido3 ELISA Kit, dio-1 ELISA Kit, iodothyronine deiodinase 1 ELISA Kit, death inducer-obliterator 1 ELISA Kit, death inducer-obliterator 1 L homeolog ELISA Kit, DIO1 ELISA Kit, DIDO1 ELISA Kit, Dido1 ELISA Kit, dido1.L ELISA Kit, dido1 ELISA Kit
    基因ID
    11083
    UniProt
    Q9BTC0
    途径
    Hormone Activity
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