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Crk ELISA 试剂盒

CRK 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN5690859
发货至: 中国
  • 抗原 See all Crk (CRK) ELISA试剂盒
    Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))
    适用
    • 3
    • 3
    • 3
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human Crk ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human Crk.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    6 months
  • 抗原 See all Crk (CRK) ELISA试剂盒
    Crk (CRK) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian) (CRK))
    别名
    Crk (CRK 产品)
    别名
    CG1587 ELISA Kit, CRK ELISA Kit, D-CRK ELISA Kit, D-Crk ELISA Kit, DCrk ELISA Kit, Dcrk ELISA Kit, Dmel\\CG1587 ELISA Kit, crk ELISA Kit, dCRK ELISA Kit, dCrk ELISA Kit, fb34h10 ELISA Kit, fc05a01 ELISA Kit, fi19g05 ELISA Kit, wu:fb34h10 ELISA Kit, wu:fc05a01 ELISA Kit, wu:fc54a04 ELISA Kit, wu:fi19g05 ELISA Kit, zgc:100936 ELISA Kit, CRKII ELISA Kit, p38 ELISA Kit, Crko ELISA Kit, crk2 ELISA Kit, crkII ELISA Kit, v-crk ELISA Kit, P38C-CRK ELISA Kit, Crk-I ELISA Kit, Crk-II ELISA Kit, Crk-III ELISA Kit, Crk3 ELISA Kit, CrkIII ELISA Kit, c-Crk ELISA Kit, Crk oncogene ELISA Kit, v-crk avian sarcoma virus CT10 oncogene homolog ELISA Kit, CRK proto-oncogene, adaptor protein ELISA Kit, v-crk avian sarcoma virus CT10 oncogene homolog L homeolog ELISA Kit, v-crk sarcoma virus CT10 oncogene homolog ELISA Kit, Crk ELISA Kit, crk ELISA Kit, CRK ELISA Kit, crk.L ELISA Kit
    基因ID
    1398
    UniProt
    P46108
    途径
    Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Signaling of Hepatocyte Growth Factor Receptor
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