Catalase ELISA 试剂盒
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Quick Overview for Catalase ELISA 试剂盒 (ABIN5690839)
抗原
See all Catalase (CAT) ELISA试剂盒适用
检测方法
实验类型
检测范围
应用范围
样品类型
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最低检测浓度
- 0.055 ng/mL
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原理
- Human Catalase ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
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Analytical Method
- Quantitative
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特异性
- This ELISA kit shows no cross-reactivity with the following cytokines tested: human 15-PGDH, ALPL, ANGPTL2, Annexin A1, Attractin, Cathepsin C, CD47, CD79A, CHIT1, CLUL1, CNDP1, CNP, COCO, ELF3, FABP3, FABP5, Fibromodulin, FLRT3, FOLR3, GFR alpha-3, GSTP1, IDS, IkB-alpha, JNK2, KLK6, KLK8, NELL1, NTH1, OBCAM, PRX1, SCCA1, Serpin A12, Sirtuin 3, SOD1, SOX10, SULT1B1, SULT1C2, SULT2B1, TIGIT.
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产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Catalase (CAT) ELISA Kit
CAT 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Catalase (CAT) ELISA KitCAT 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Catalase (CAT) ELISA KitCAT 适用: 人 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Catalase (CAT) ELISA KitCAT 适用: 小鼠 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Catalase (CAT) ELISA KitCAT 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Catalase (CAT) ELISA KitCAT 适用: 大鼠 Colorimetric Sandwich ELISA 93.75 mIU/mL - 6000 mIU/mL Cell Culture Supernatant, Plasma, Serum
Catalase (CAT) ELISA KitCAT 适用: 鱼 Colorimetric Competition ELISA 125 mU/mL - 2000 mU/mL Plasma, Serum
Catalase (CAT) ELISA KitCAT 适用: 马 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Catalase (CAT) ELISA KitCAT 适用: 小鸡 Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Catalase (CAT) ELISA KitCAT 适用: 大鼠 Colorimetric Sandwich ELISA 31.25 mIU/mL - 2000 mIU/mL Plasma, Serum, Tissue Homogenate
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应用备注
- Suggested Plasma Dilution: 50 fold
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- -20 °C
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储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
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有效期
- 6 months
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- Catalase (CAT)
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别名
- Catalase
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背景
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Gene Symbols: CAT
Protein Name (Synonyms): Catalase (EC 1.11.1.6)
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基因ID
- 847
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UniProt
- P04040
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途径
- Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism
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