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Catalase ELISA 试剂盒

CAT 适用: 人 Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
产品编号 ABIN5690839
发货至: 中国
  • 抗原 See all Catalase (CAT) ELISA试剂盒
    Catalase (CAT)
    适用
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    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    应用范围
    ELISA
    原理
    Human Catalase ELISA Kit.
    样品类型
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    特异性
    This ELISA antibody pair recognizes Human Catalase.
    产品特性
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    组件
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    试剂未包括
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • 板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    6 months
  • 抗原 See all Catalase (CAT) ELISA试剂盒
    Catalase (CAT)
    别名
    Catalase (CAT 产品)
    别名
    2210418N07 ELISA Kit, Cas-1 ELISA Kit, Cas1 ELISA Kit, Cs-1 ELISA Kit, CS1 ELISA Kit, Cat01 ELISA Kit, Catl ELISA Kit, fb68a12 ELISA Kit, wu:fb68a12 ELISA Kit, CAT ELISA Kit, CATA ELISA Kit, CG6871 ELISA Kit, CT21282 ELISA Kit, CatA ELISA Kit, DMCATHPO ELISA Kit, DROCATHPO ELISA Kit, Dmel\\CG6871 ELISA Kit, U00145 ELISA Kit, bs36h11.y1 ELISA Kit, cat ELISA Kit, GB11648 ELISA Kit, catalase ELISA Kit, Cat ELISA Kit, BA0843 ELISA Kit, DKFZp469E0232 ELISA Kit, catalase ELISA Kit, Catalase ELISA Kit, catalase, gene 2 ELISA Kit, CAT ELISA Kit, Cat ELISA Kit, cat ELISA Kit, cat.2 ELISA Kit, LOC769804 ELISA Kit, BA_0843 ELISA Kit
    基因ID
    847
    UniProt
    P04040
    途径
    Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism
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