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APP ELISA 试剂盒

This Colorimetric ELISA kit is designed for the quantitative measurement of 人 APP.

产品编号 ABIN5690821

发货至: 中国

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中国
北京 101111

No. 88 KeChuang 6th Street

Beijing Economic Technological Development Area

Room 801-803

4A Biotech Co.,Ltd.

Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for APP ELISA 试剂盒 (ABIN5690821)

抗原

See all APP ELISA试剂盒

APP (Amyloid beta (A4) Precursor Protein (APP))

适用

人

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

0.18 ng/mL - 150 ng/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Plasma, Serum

Discover our top product APP ELISA Kit

最低检测浓度

0.18 ng/mL

原理

Human Amyloid-beta A4 protein/APP/PN-II ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Analytical Method

Quantitative

特异性

This ELISA antibody pair detects human APP. Other species not determined.

产品特性
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
组件
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
试剂未包括
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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应用备注

Suggested Plasma Dilution: 3 - 20 fold

板类型

Pre-coated

实验流程
1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.
限制

仅限研究用
储存条件

-20 °C

储存方法

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

有效期

6 months
抗原 See all APP ELISA试剂盒

APP (Amyloid beta (A4) Precursor Protein (APP))

别名

Amyloid-beta A4 protein (APP/PN-II)

背景

Gene Symbols: APP, A4, AD1
Protein Name (Synonyms): Amyloid beta A4 protein (ABPP) (APPI) (APP) (Alzheimer disease amyloid protein) (Amyloid precursor protein) (Beta-amyloid precursor protein) (Cerebral vascular amyloid peptide) (CVAP) (PreA4) (Protease nexin-II) (PN-II) [Cleaved into: N-APP Soluble APP-alpha (S-APP-alpha) Soluble APP-beta (S-APP-beta) C99 Beta-amyloid protein 42 (Beta-APP42) Beta-amyloid protein 40 (Beta-APP40) C83 P3(42) P3(40) C80 Gamma-secretase C-terminal fragment 59 (Amyloid intracellular domain 59) (AICD-59) (AID(59)) (Gamma-CTF(59)) Gamma-secretase C-terminal fragment 57 (Amyloid intracellular domain 57) (AICD-57) (AID(57)) (Gamma-CTF(57)) Gamma-secretase C-terminal fragment 50 (Amyloid intracellular domain 50) (AICD-50) (AID(50)) (Gamma-CTF(50)) C31

基因ID

351

UniProt

P05067

途径

Caspase Cascade in Apoptosis, EGFR Signaling Pathway, Transition Metal Ion Homeostasis, Skeletal Muscle Fiber Development, Toll-Like Receptors Cascades, Feeding Behaviour