PPARA ELISA 试剂盒
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- 抗原 See all PPARA ELISA试剂盒
- PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- DNA-Binding ELISA
- 应用范围
- ELISA
- 原理
- Human PPAR-alpha Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical PPAR-alpha binding sequences to semi-quantitatively detect active PPAR-alpha in lysates or nuclear extracts.
- 样品类型
- Cell Lysate, Nuclear Extract
- Analytical Method
- Semi-Quantitative
- 特异性
- The olionucleotide/antibody pair provided in this kit recognizes human PPAR-alpha in whole lysates and nuclear extracts.
- 产品特性
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- Specific transcription factor-DNA binding assay
- Perfect alternative to EMSA
- Easy to perform in an ELISA format
- Non-radioactive assay
- High throughput (96 well plate format)
- Assay can be completed within 5 hours
- 组件
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- 96-well Strip Microplate pre-coated with DNA probes
- DNA Binding Buffer
- Positive Control Sample
- Specific Competitor DNA probe
- Non-specific Competitor DNA probe
- Assay Reagent
- DTT
- Wash Buffer
- Primary Antibody
- HRP-conjugated Secondary Antibody
- TMB One-Step Substrate Reagent
- Stop Solution
- 试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions Absorbent paper
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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- Discover our top product PPARA ELISA Kit
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Peroxisome Proliferator-Activated Receptor alpha (PPARA) ELISA Kit
PPARA 适用: 人 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor alpha (PPARA) ELISA KitPPARA 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor alpha (PPARA) ELISA KitPPARA 适用: 大鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor alpha (PPARA) ELISA KitPPARA 适用: 人 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Peroxisome Proliferator-Activated Receptor alpha (PPARA) ELISA KitPPARA 适用: 小鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared HRP-secondary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 储存方法
- Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
- 有效期
- 6 months
-
- 抗原 See all PPARA ELISA试剂盒
- PPARA (Peroxisome Proliferator-Activated Receptor alpha (PPARA))
- 别名
- PPAR-alpha (PPARA 产品)
- 别名
- NR1C1 ELISA Kit, PPAR ELISA Kit, PPARalpha ELISA Kit, hPPAR ELISA Kit, LOC100136415 ELISA Kit, PPARALPHA ELISA Kit, nr1c1 ELISA Kit, xPPARalpha ELISA Kit, xppara ELISA Kit, 4933429D07Rik ELISA Kit, AW742785 ELISA Kit, Nr1c1 ELISA Kit, PPAR-alpha ELISA Kit, Ppar ELISA Kit, peroxisome proliferator activated receptor alpha ELISA Kit, peroxisome proliferator-activated receptor alpha ELISA Kit, peroxisome proliferator activated receptor alpha L homeolog ELISA Kit, PPARA ELISA Kit, LOC100136415 ELISA Kit, ppara.L ELISA Kit, Ppara ELISA Kit, ppara ELISA Kit
- 基因ID
- 5465
- UniProt
- Q07869
- 途径
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha, Regulation of Carbohydrate Metabolic Process, Hepatitis C
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