GATA1 ELISA 试剂盒
This 人 GATA1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify 人 GATA1 in Cell Lysate 和 Nuclear Extract.
Quick Overview for GATA1 ELISA 试剂盒 (ABIN5690754)
抗原
See all GATA1 ELISA试剂盒
GATA1
(GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))
适用
人
检测方法
Colorimetric
实验类型
DNA-Binding ELISA
应用范围
ELISA
样品类型
Cell Lysate, Nuclear Extract
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原理
- Human GATA-1 Transcription Factor Activity Assay. This assay uses a dsDNA coated plate with canonical GATA1 binding sequences to semi-quantitatively detect active GATA1 in lysates or nuclear extracts.
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Analytical Method
- Semi-Quantitative
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特异性
- The olionucleotide/antibody pair provided in this kit recognizes human GATA-1 in whole lysates and nuclear extracts.
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产品特性
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- Specific transcription factor-DNA binding assay
- Perfect alternative to EMSA
- Easy to perform in an ELISA format
- Non-radioactive assay
- High throughput (96 well plate format)
- Assay can be completed within 5 hours
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组件
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- 96-well Strip Microplate pre-coated with DNA probes
- DNA Binding Buffer
- Positive Control Sample
- Specific Competitor DNA probe
- Non-specific Competitor DNA probe
- Assay Reagent
- DTT
- Wash Buffer
- Primary Antibody
- HRP-conjugated Secondary Antibody
- TMB One-Step Substrate Reagent
- Stop Solution
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试剂未包括
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- Distilled or deionized water
- 100 mL and 1 liter graduated cylinders
- Tubes to prepare sample dilutions
- Absorbent paper
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- Benchtop rocker or shaker
- Microplate reader capable of measuring absorbance at 450 nm
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板类型
- Pre-coated
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实验流程
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- Prepare all reagents and samples as instructed in the manual.
- Add 100 μL of sample or positive control to each well.
- Incubate 2 h at RT or O/N at 4 °C.
- Add 100 μL of prepared primary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared HRP-secondary antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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限制
- 仅限研究用
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储存条件
- 4 °C
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储存方法
- Upon receipt, the positive control should be removed and stored at -20° or -80°C. The remainder of the kit can be stored for up to 6 months at 2-8°C from the date of shipment. Opened Microplate Wells or reagents may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: The kit can be used within one year if the whole kit is stored at -20°C upon receipt. Avoid repeated freeze-thaw cycles.
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有效期
- 6 months
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- GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))
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别名
- GATA1
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基因ID
- 2623
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UniProt
- P15976
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途径
- Cellular Response to Molecule of Bacterial Origin
抗原 See all GATA1 ELISA试剂盒
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