SDHA ELISA 试剂盒
SDHA
适用: 大鼠
Colorimetric
Sandwich ELISA
78.125 pg/mL - 5000 pg/mL
Tissue Homogenate
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- 抗原 See all SDHA ELISA试剂盒
- SDHA (Succinate Dehydrogenase Complex, Subunit A, Flavoprotein (Fp) (SDHA))
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适用
- 大鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 78.125 pg/mL - 5000 pg/mL
- 最低检测浓度
- 78.125 pg/mL
- 应用范围
- ELISA
- 样品类型
- Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 31 pg/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Top Product
- Discover our top product SDHA ELISA Kit
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Succinate Dehydrogenase Complex, Subunit A, Flavoprotein (Fp) (SDHA) ELISA Kit
SDHA 适用: 小鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
-
- 抗原 See all SDHA ELISA试剂盒
- SDHA (Succinate Dehydrogenase Complex, Subunit A, Flavoprotein (Fp) (SDHA))
- 别名
- Succinate Dehydrogenase Complex Subunit A (SDHA) (SDHA 产品)
- 别名
- CG17246 ELISA Kit, Dmel\\CG17246 ELISA Kit, Scs-fp ELISA Kit, Sdh ELISA Kit, Sdha ELISA Kit, scs-fp ELISA Kit, sdh ELISA Kit, CMD1GG ELISA Kit, FP ELISA Kit, PGL5 ELISA Kit, SDH1 ELISA Kit, SDH2 ELISA Kit, SDHF ELISA Kit, 1500032O14Rik ELISA Kit, 2310034D06Rik ELISA Kit, 4921513A11 ELISA Kit, C81073 ELISA Kit, im:7141001 ELISA Kit, zgc:56051 ELISA Kit, Fp ELISA Kit, sdha-b ELISA Kit, Succinate dehydrogenase, subunit A (flavoprotein) ELISA Kit, succinate dehydrogenase complex flavoprotein subunit A ELISA Kit, succinate dehydrogenase complex, subunit A, flavoprotein (Fp) ELISA Kit, succinate dehydrogenase complex subunit A, flavoprotein (Fp) S homeolog ELISA Kit, SdhA ELISA Kit, SDHA ELISA Kit, Sdha ELISA Kit, sdha ELISA Kit, sdha.S ELISA Kit
- 背景
- Alternative name: SDH2, SDHF, FP, Flavoprotein, Flavoprotein subunit of complex II, Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
- 基因ID
- 157074
- UniProt
- Q920L2
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