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PAX6 ELISA 试剂盒

PAX6 适用: 小鼠 Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Tissue Homogenate
产品编号 ABIN5674917
发货至: 中国
  • 抗原 See all PAX6 ELISA试剂盒
    PAX6 (Paired Box 6 (PAX6))
    适用
    • 1
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.312 ng/mL - 20 ng/mL
    最低检测浓度
    0.312 ng/mL
    应用范围
    ELISA
    样品类型
    Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    0124 ng/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all PAX6 ELISA试剂盒
    PAX6 (Paired Box 6 (PAX6))
    别名
    Paired Box Gene 6 (PAX6) (PAX6 产品)
    别名
    pax6 ELISA Kit, an2 ELISA Kit, mgda ELISA Kit, wagr ELISA Kit, pax-6 ELISA Kit, pax6a ELISA Kit, xpax6 ELISA Kit, XLPAX6 ELISA Kit, MGC52531 ELISA Kit, PAX-6 ELISA Kit, AN ELISA Kit, AN2 ELISA Kit, D11S812E ELISA Kit, MGDA ELISA Kit, WAGR ELISA Kit, Pax-6 ELISA Kit, pax6b ELISA Kit, 1500038E17Rik ELISA Kit, AEY11 ELISA Kit, Dey ELISA Kit, Gsfaey11 ELISA Kit, Sey ELISA Kit, PAXZF-A ELISA Kit, Pax6.1 ELISA Kit, cb280 ELISA Kit, fc20e07 ELISA Kit, pax-a ELISA Kit, pax[zf-a] ELISA Kit, paxzfa ELISA Kit, wu:fc20e07 ELISA Kit, zfpax-6a ELISA Kit, zfpax-6b ELISA Kit, paired box 6 ELISA Kit, paired box 6 L homeolog ELISA Kit, paired box 6 S homeolog ELISA Kit, paired box 6a ELISA Kit, PAX6 ELISA Kit, pax6 ELISA Kit, Pax6 ELISA Kit, pax6.L ELISA Kit, pax6.S ELISA Kit, pax6a ELISA Kit
    背景
    Alternative name: AN, AN2, MGDA, WAGR, Oculorhombin, Aniridia,Keratitis, Aniridia type II protein
    基因ID
    18508
    UniProt
    P63015
    途径
    Peptide Hormone Metabolism, Carbohydrate Homeostasis
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