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HMGCR ELISA 试剂盒

HMGCR 适用: 小鼠 Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Cell Lysate, Tissue Homogenate
产品编号 ABIN5674904
发货至: 中国
  • 抗原 See all HMGCR ELISA试剂盒
    HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.781 ng/mL - 50 ng/mL
    最低检测浓度
    0.781 ng/mL
    应用范围
    ELISA
    样品类型
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    0.31 ng/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all HMGCR ELISA试剂盒
    HMGCR (3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGCR))
    别名
    3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase (HMGCR) (HMGCR 产品)
    别名
    HMGR ELISA Kit, LDLCQ3 ELISA Kit, hmg-coa-r ELISA Kit, HMG-CoAR ELISA Kit, Red ELISA Kit, 3H3M ELISA Kit, BcDNA:LD15354 ELISA Kit, CG10367 ELISA Kit, Clb ELISA Kit, DmHMG-CoAr ELISA Kit, Dmel\\CG10367 ELISA Kit, HMGCR ELISA Kit, HMGCR_Dm ELISA Kit, HMGCoAR ELISA Kit, HMGCoARr ELISA Kit, HMGCoAr ELISA Kit, HmG-CoAR ELISA Kit, Hmg ELISA Kit, bro1 ELISA Kit, bs32f05.y1 ELISA Kit, clb ELISA Kit, hmgc ELISA Kit, hmgcr ELISA Kit, hmgrc ELISA Kit, l(3)01152 ELISA Kit, l(3)04684 ELISA Kit, hmgcr2 ELISA Kit, im:6912716 ELISA Kit, zgc:110746 ELISA Kit, 3-hydroxy-3-methylglutaryl-CoA reductase ELISA Kit, 3-hydroxy-3-methylglutaryl-CoA reductase L homeolog ELISA Kit, 3-hydroxy-3-methylglutaryl-Coenzyme A reductase ELISA Kit, HMG Coenzyme A reductase ELISA Kit, 3-hydroxy-3-methylglutaryl-CoA reductase b ELISA Kit, HMGCR ELISA Kit, hmgcr.L ELISA Kit, Hmgcr ELISA Kit, hmgcrb ELISA Kit
    背景
    Alternative name: HMG-CoA Reductase, Hydroxymethylglutaryl-CoA Reductase
    基因ID
    15357
    UniProt
    Q01237
    途径
    AMPK Signaling, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha
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