PDE3A ELISA 试剂盒
This 人 PDE3A ELISA Kit specifically detects PDE3A
in Tissue Homogenate.
产品编号 ABIN5674894
发货至:
中国
Contact our Customer Service for availability and price in your country.
Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
Quick Overview for PDE3A ELISA 试剂盒 (ABIN5674894)
抗原
See all PDE3A ELISA试剂盒
PDE3A
(phosphodiesterase 3A, cGMP-Inhibited (PDE3A))
适用
人
检测方法
Colorimetric
实验类型
Sandwich ELISA
检测范围
0.312 ng/mL - 20 ng/mL
应用范围
ELISA
样品类型
Tissue Homogenate
-
-
最低检测浓度
- 0.312 ng/mL
-
Analytical Method
- Quantitative
-
灵敏度
- 0101 ng/mL
-
组件
-
Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual -
试剂未包括
-
1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.
-
-
phosphodiesterase 3A, cGMP-Inhibited (PDE3A) ELISA Kit
PDE3A 适用: 大鼠 Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Tissue Homogenate
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
实验时间
- 1 - 4.5 h
-
板类型
- Pre-coated
-
实验流程
-
1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. -
限制
- 仅限研究用
-
-
-
储存条件
- 4 °C
-
有效期
- 12 months
-
-
- PDE3A (phosphodiesterase 3A, cGMP-Inhibited (PDE3A))
-
别名
- Phosphodiesterase 3A, cGMP Inhibited (PDE3A)
-
背景
- Alternative name: CGI-PDE, Cyclic GMP-inhibited phosphodiesterase A, cGMP-inhibited 3',5'-cyclic phosphodiesterase A
-
基因ID
- 5139
-
UniProt
- Q14432
-
途径
- cAMP Metabolic Process
抗原 See all PDE3A ELISA试剂盒
-
You are here:
