GSTA3 ELISA 试剂盒
-
- 抗原 See all GSTA3 ELISA试剂盒
- GSTA3 (Glutathione S-Transferase alpha 3 (GSTA3))
-
适用
- 小鼠
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 0049 ng/mL
- 组件
-
Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
-
1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Top Product
- Discover our top product GSTA3 ELISA Kit
-
Glutathione S-Transferase alpha 3 (GSTA3) ELISA Kit
GSTA3 适用: 人 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Glutathione S-Transferase alpha 3 (GSTA3) ELISA KitGSTA3 适用: 大鼠 Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
-
- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
-
1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
-
- 储存条件
- 4 °C
- 有效期
- 12 months
-
- 抗原 See all GSTA3 ELISA试剂盒
- GSTA3 (Glutathione S-Transferase alpha 3 (GSTA3))
- 别名
- Glutathione S Transferase Alpha 3 (GSTa3) (GSTA3 产品)
- 别名
- GSTA3 ELISA Kit, GST ELISA Kit, GSTA1 ELISA Kit, GSTA3-3 ELISA Kit, GTA3 ELISA Kit, GSTA2 ELISA Kit, Gst2-3 ELISA Kit, Yc2 ELISA Kit, glutathione S-transferase alpha 3 ELISA Kit, glutathione S-transferase A2 ELISA Kit, glutathione S-transferase ELISA Kit, glutathione S-transferase, alpha 3 ELISA Kit, glutathione S-transferase Yc ELISA Kit, GSTA3 ELISA Kit, LOC474938 ELISA Kit, gsta3 ELISA Kit, Gsta3 ELISA Kit, LOC100328911 ELISA Kit
- 背景
- Alternative name: GST-A3, GSTA3-3, GTA3, GST class-alpha member 3
- 基因ID
- 14859
- UniProt
- P30115
-
