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ZEB2 ELISA 试剂盒

ZEB2 适用: 人 Colorimetric Sandwich ELISA 1.25 ng/mL - 80 ng/mL Tissue Homogenate
产品编号 ABIN5674882
发货至: 中国
  • 抗原 See all ZEB2 ELISA试剂盒
    ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))
    适用
    • 4
    • 3
    • 2
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    1.25 ng/mL - 80 ng/mL
    最低检测浓度
    1.25 ng/mL
    应用范围
    ELISA
    样品类型
    Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    0.6 ng/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all ZEB2 ELISA试剂盒
    ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))
    别名
    Zinc Finger Homeobox Protein 1B (ZFHX1B) (ZEB2 产品)
    别名
    ZFHX1B ELISA Kit, Zfhx1b ELISA Kit, HSPC082 ELISA Kit, SIP-1 ELISA Kit, SIP1 ELISA Kit, SMADIP1 ELISA Kit, 9130203F04Rik ELISA Kit, D130016B08Rik ELISA Kit, Zfx1b ELISA Kit, Zfxh1b ELISA Kit, mKIAA0569 ELISA Kit, XSip1 ELISA Kit, sip1 ELISA Kit, zfhx1b ELISA Kit, si:dkey-52o2.2 ELISA Kit, sip1a ELISA Kit, wu:fc92g08 ELISA Kit, sip1b ELISA Kit, zinc finger E-box binding homeobox 2 ELISA Kit, zinc finger E-box binding homeobox 2 S homeolog ELISA Kit, zinc finger homeobox protein ELISA Kit, zinc finger E-box binding homeobox 2a ELISA Kit, zinc finger E-box binding homeobox 2b ELISA Kit, ZEB2 ELISA Kit, Zeb2 ELISA Kit, zeb2.S ELISA Kit, zeb2 ELISA Kit, zeb2a ELISA Kit, zeb2b ELISA Kit
    背景
    Alternative name: ZEB2, SIP1, SIP-1, SMADIP1, SIP1, Zinc Finger E-Box Binding Homeobox 2, SMAD Interacting Protein 1
    基因ID
    9839
    UniProt
    O60315
    途径
    Tube Formation
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