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ZEB2 ELISA 试剂盒

This 人 ZEB2 ELISA Kit specifically detects ZEB2 in Tissue Homogenate.
产品编号 ABIN5674882
发货至: 中国
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中国
北京 101111
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Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for ZEB2 ELISA 试剂盒 (ABIN5674882)

抗原

See all ZEB2 ELISA试剂盒
ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

适用

  • 4
  • 3
  • 2

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

1.25 ng/mL - 80 ng/mL

应用范围

ELISA

样品类型

Tissue Homogenate
  • 最低检测浓度

    1.25 ng/mL

    Analytical Method

    Quantitative

    灵敏度

    0.6 ng/mL

    组件

    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual

    试剂未包括

    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
  • 应用备注

    Optimal working dilution should be determined by the investigator.

    实验时间

    1 - 4.5 h

    板类型

    Pre-coated

    实验流程

    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.

    限制

    仅限研究用
  • 储存条件

    4 °C

    有效期

    12 months
  • 抗原 See all ZEB2 ELISA试剂盒

    ZEB2 (Zinc Finger E-Box Binding Homeobox 2 (ZEB2))

    别名

    Zinc Finger Homeobox Protein 1B (ZFHX1B)

    背景

    Alternative name: ZEB2, SIP1, SIP-1, SMADIP1, SIP1, Zinc Finger E-Box Binding Homeobox 2, SMAD Interacting Protein 1

    基因ID

    9839

    UniProt

    O60315

    途径

    Tube Formation
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