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AQP2 ELISA 试剂盒

AQP2 适用: 小鼠 Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
产品编号 ABIN5674870
发货至: 中国
  • 抗原 See all AQP2 ELISA试剂盒
    AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))
    适用
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    小鼠
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    0.156 ng/mL - 10 ng/mL
    最低检测浓度
    0.156 ng/mL
    应用范围
    ELISA
    样品类型
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    0059 ng/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
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  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all AQP2 ELISA试剂盒
    AQP2 (Aquaporin 2 (Collecting Duct) (AQP2))
    别名
    Aquaporin 2, Collecting Duct (AQP2) (AQP2 产品)
    别名
    AQP-CD ELISA Kit, WCH-CD ELISA Kit, cph ELISA Kit, jpk ELISA Kit, AQP-2 ELISA Kit, aquaporin-2 ELISA Kit, aquaporin 2 ELISA Kit, aquaporin 2 (collecting duct) ELISA Kit, Aqp2 ELISA Kit, AQP2 ELISA Kit
    背景
    Alternative name: AQP-CD, WCH-CD, ADH water channel, Aquaporin-CD, Collecting duct water channel protein, Water channel protein for renal collecting duct
    基因ID
    11827
    UniProt
    P56402
    途径
    Response to Water Deprivation
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