PYCARD ELISA 试剂盒
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- 抗原 See all PYCARD ELISA试剂盒
- PYCARD (PYD and CARD Domain Containing (PYCARD))
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适用
- 人
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 0.156 ng/mL - 10 ng/mL
- 最低检测浓度
- 0.156 ng/mL
- 应用范围
- ELISA
- 样品类型
- Plasma, Serum, Tissue Homogenate
- Analytical Method
- Quantitative
- 灵敏度
- 0065 ng/mL
- 组件
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Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual - 试剂未包括
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1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution. - Featured
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PYD and CARD Domain Containing (PYCARD) ELISA Kit
PYCARD 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
PYD and CARD Domain Containing (PYCARD) ELISA KitPYCARD 适用: 小鼠 Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
PYD and CARD Domain Containing (PYCARD) ELISA KitPYCARD 适用: 小鼠 Colorimetric Sandwich ELISA 7.81 pg/mL - 500 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
PYD and CARD Domain Containing (PYCARD) ELISA KitPYCARD 适用: 大鼠 Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 实验时间
- 1 - 4.5 h
- 板类型
- Pre-coated
- 实验流程
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1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately. - 限制
- 仅限研究用
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- 储存条件
- 4 °C
- 有效期
- 12 months
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- 抗原 See all PYCARD ELISA试剂盒
- PYCARD (PYD and CARD Domain Containing (PYCARD))
- 别名
- PYD And CARD Domain Containing Protein (PYCARD) (PYCARD 产品)
- 别名
- ASC ELISA Kit, CARD5 ELISA Kit, TMS ELISA Kit, TMS-1 ELISA Kit, TMS1 ELISA Kit, 9130417A21Rik ELISA Kit, Asc ELISA Kit, TNS1 ELISA Kit, masc ELISA Kit, asc1 ELISA Kit, zAsc ELISA Kit, zgc:109874 ELISA Kit, PYD and CARD domain containing ELISA Kit, PYCARD ELISA Kit, Pycard ELISA Kit, pycard ELISA Kit
- 背景
- Alternative name: ASC, CARD5, TMS1, Apoptosis-associated speck-like protein containing a CARD, Caspase recruitment domain-containing protein 5, Target of methylation-induced silencing 1
- 基因ID
- 29108
- UniProt
- Q9ULZ3
- 途径
- Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome
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