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PAOX ELISA 试剂盒

PAOX 适用: 人 Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
产品编号 ABIN5674857
发货至: 中国
  • 抗原 See all PAOX ELISA试剂盒
    PAOX (Polyamine Oxidase (Exo-N4-Amino) (PAOX))
    适用
    • 1
    • 1
    检测方法
    Colorimetric
    实验类型
    Sandwich ELISA
    检测范围
    78.125 pg/mL - 5000 pg/mL
    最低检测浓度
    78.125 pg/mL
    应用范围
    ELISA
    样品类型
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    灵敏度
    31.5 pg/mL
    组件
    Pre-coated, ready to use 96-well strip plate
    Standard (freeze dried)
    Standard Diluent
    Detection Reagent A
    Detection Reagent B
    Assay Diluent A
    Assay Diluent B
    TMB Substhumane
    Stop Solution
    Wash Buffer(30 x concenthumane)
    Plate sealer for 96 wells
    Instruction manual
    试剂未包括
    1. Microplate reader with 450 ± 10nm filter.
    2. Precision single or multi-channel pipettes and disposable tips.
    3. Eppendorf Tubes for diluting samples.
    4. Deionized or distilled water.
    5. Absorbent paper for blotting the microtiter plate.
    6. Container for Wash Solution.
    Top Product
    Discover our top product PAOX ELISA Kit
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    实验时间
    1 - 4.5 h
    板类型
    Pre-coated
    实验流程
    1. Prepare all reagents, samples and standards
    2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
    3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
    4. Aspirate and wash 3 times
    5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
    6. Aspirate and wash 5 times
    7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
    8. Add 50µL Stop Solution. Read at 450nm immediately.
    限制
    仅限研究用
  • 储存条件
    4 °C
    有效期
    12 months
  • 抗原 See all PAOX ELISA试剂盒
    PAOX (Polyamine Oxidase (Exo-N4-Amino) (PAOX))
    别名
    Polyamine Oxidase (PAOX) (PAOX 产品)
    别名
    PAO ELISA Kit, mpao1 ELISA Kit, 2410012F02Rik ELISA Kit, AI118225 ELISA Kit, Pao ELISA Kit, pao ELISA Kit, polyamine oxidase ELISA Kit, polyamine oxidase 1 ELISA Kit, amine oxidase family protein ELISA Kit, si:dkey-275b16.2 ELISA Kit, polyamine oxidase (exo-N4-amino) ELISA Kit, polyamine oxidase (exo-N4-amino) L homeolog ELISA Kit, PAOX ELISA Kit, pao1 ELISA Kit, NFIA_077590 ELISA Kit, POPTR_0011s12590g ELISA Kit, si:dkey-275b16.2 ELISA Kit, Paox ELISA Kit, paox.L ELISA Kit
    背景
    Alternative name: PAO, Peroxisomal N(1)-Acetyl-Spermine/Spermidine Oxidase(Exo-N4-Amino)
    基因ID
    196743
    UniProt
    Q6QHF9
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